TRIM36 抗体 (SS-03): sc-100881

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- TRIM36 抗体 SS-03 是小鼠单克隆 IgG₁ κ，TRIM36抗体, 在2篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组TRIM36
- 推荐用于 human 来源的TRIM36 WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取TRIM36 (SS-03): sc-100881的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成TRIM36 抗体 (SS-03)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 TRIM 抗体包括 TRIM16, TRIM23, TRIM26, TRIM3, TRIM32, TRIM35, TRIM36, TRIM37, TRIM49和 TRIM5 α
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

TRIM36 Antibody (SS-03) - Immunohistochemistry - Image 36202

TRIM36 Antibody (SS-03) - Western Blotting - Image 34253

TRIM36 Antibody (SS-03) - Immunofluorescence - Image 35711

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TRIM36 Antibody (SS-03) is an IgG₁ κ mouse monoclonal TRIM36 antibody that detects the TRIM36 protein of human origin by WB, IP, IF, IHC(P) and ELISA. TRIM36 Antibody (SS-03) is available as the non-conjugated anti-TRIM36 antibody. TRIM36 (tripartite motif-containing 36), also known as RNF98 (RING finger protein 98), HAPRIN (haploid germ cell-specific RBCC protein) or RBCC728, is a 728 amino acid protein that belongs to the TRIM/RBCC (Ring finger, B box, coiled-coil) family. Predominantly expressed in prostate, testis and brain with weak expression in heart, kidney and lung, TRIM36 contains two B box-type zinc fingers, a SPRY domain, a coiled-coil domain, a fibronectin type-III domain and a RING-type zinc finger; a motif that has zinc-chelating activity and is involved in mediating protein-protein and protein-DNA interactions. Localizing to the cytoplasm and the acrosomal region of germ cells and mature sperm, TRIM36 is believed to play a role in the acrosome reaction and fertilization. In addition, TRIM36 is overexpressed in prostate cancer, suggesting a possible role for TRIM36 in prostate tumorigenesis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

The tripartite motif family identifies cell compartments. | Reymond, A., et al. 2001. EMBO J. 20: 2140-51. PMID: 11331580
Haprin, a novel haploid germ cell-specific RING finger protein involved in the acrosome reaction. | Kitamura, K., et al. 2003. J Biol Chem. 278: 44417-23. PMID: 12917430
Cloning and characterisation of the RBCC728/TRIM36 zinc-binding protein from the tumor suppressor gene region at chromosome 5q22.3. | Balint, I., et al. 2004. Gene. 332: 45-50. PMID: 15145053
Identification of human HAPRIN potentially involved in the acrosome reaction. | Kitamura, K., et al. 2005. J Androl. 26: 511-8. PMID: 15955891
The RING-finger protein haprin: domains and function in the acrosome reaction. | Kitamura, K., et al. 2005. Curr Protein Pept Sci. 6: 567-74. PMID: 16381605
Subclassification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding. | Short, KM. and Cox, TC. 2006. J Biol Chem. 281: 8970-80. PMID: 16434393

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	TRIM36	Entrez Gene ID: 55521	5q22.3	XM_017009621, XM_017009622, XM_017009623, NM_001017397, NM_001017398, NM_001300752, NM_001300759, NM_018700	UniProt ID: Q9NQ86	609317

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗TRIM36抗体(SS-03)在各种科学出版物中有2篇引文。

针对哺乳动物靶标蛋白的一抗，如抗TRIM36 抗体 (SS-03)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

TRIM36 抗体 (SS-03) Specifications

替代产品名称

TRIM36

产品克隆名称

SS-03

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

391-500

抗原表位靶标

TRIM36

抗原表位种属

human

产品反应性/检测

TRIM36

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

2篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗TRIM36抗体(SS-03)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

TRIM36 has 5 isoform. Can this siRNA knock down all isoforms?

Asked by: Aycan

Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.

Answered by: Anonymous

Date published: 2023-03-13

Can sc-100881: TRIM36 (SS-03) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt

Thank you for your question. Yes, sc-100881: TRIM36 (SS-03) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 4 out of 5 by QZ from Precise signal with some background achieved Precise signal with some background achieved using IMR-32 whole cell lysate. -SCBT QC

Date published: 2015-06-20

Rated 3 out of 5 by FL from Produced satisfactory immunoperoxidase cytoplasmic Produced satisfactory immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human pancreas tissue. -SCBT QC

Date published: 2015-03-29

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TRIM36 抗体 (SS-03): sc-100881

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References:

TRIM36 抗体 (SS-03) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

TRIM36 has 5 isoform. Can this siRNA knock down all isoforms?

Can sc-100881: TRIM36 (SS-03) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?