Tom34 抗体 (S-05): sc-101284

Tom34 Antibody (S-05) is an IgG_2a κ mouse monoclonal Tom34 antibody (also designated TOMM34 antibody) that detects the Tom34 protein of human origin by WB, IP, IF and ELISA. Tom34 Antibody (S-05) is available as the non-conjugated anti-Tom34 antibody. The mitochondrial preprotein translocases of the outer membrane (Tom) form a multisubunit complex that facilitates the import of nuclear-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins, and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondrion. Tom34 (translocase of outer mitochondrial membrane 34), also known as TOMM34, URCC3 or HTOM34P, is a ubiquitously expressed 309 amino acid protein that contains six TPR repeats. Localized to the cytoplasmic side of the mitochondrion, Tom34 functions as a chaperone-like protein that binds the mature portion of unfolded proteins and guides their import into mitochondria. In addition, Tom34 is thought to have weak ATPase activity and may interact with other organelles within the cell. Expression of Tom34 is upregulated in colon cancer cells and is thought to be involved in tumor progression. Tom34 may, therefore, be a novel target for therapeutic anti-cancer drugs.

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References:

1. Characterization of the novel mitochondrial protein import component, Tom34, in mammalian cells.  |  Chewawiwat, N., et al. 1999. J Biochem. 125: 721-7. PMID: 10101285
2. Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins.  |  Mukhopadhyay, A., et al. 2002. Arch Biochem Biophys. 400: 97-104. PMID: 11913975
3. Yeast two-hybrid screening identifies binding partners of human Tom34 that have ATPase activity and form a complex with Tom34 in the cytosol.  |  Yang, CS. and Weiner, H. 2002. Arch Biochem Biophys. 400: 105-10. PMID: 11913976
4. Conformation-sensitive gel electrophoresis as an ideal high-throughput strategy for accurate detection of sequence variations in DNA: screening hTomm and hTimm genes.  |  Blesa, JR., et al. 2004. J Biomol Screen. 9: 621-4. PMID: 15475482
5. Identification of TOMM34, which shows elevated expression in the majority of human colon cancers, as a novel drug target.  |  Shimokawa, T., et al. 2006. Int J Oncol. 29: 381-6. PMID: 16820880
6. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.  |  Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
7. NRF-1 is the major transcription factor regulating the expression of the human TOMM34 gene.  |  Blesa, JR., et al. 2008. Biochem Cell Biol. 86: 46-56. PMID: 18364745
8. hTom34: a novel translocase for the import of proteins into human mitochondria.  |  Nuttall, SD., et al. 1997. DNA Cell Biol. 16: 1067-74. PMID: 9324309