Tom22 抗体 (J-31): sc-101286

Tom22 Antibody (J-31) is an IgG2a κ mouse monoclonal Tom22 antibody that detects Tom22 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Tom22 Antibody (J-31) is available as the non-conjugated anti-Tom22 antibody form. The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex, as it functions as a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP (5,6). Tom22 directly associates with Tom40, the major component of the GIP, and forms a stable interaction between the two core complexes. This interactions facilitates the fluid movement of preproteins into the mitochondria. Structural features of Tom22 include an N-terminal negatively charged region exposed to the cytosol, a C-terminal innermembrane space region with little negative charge, and a putative transmembrane region. The gene encoding human Tom22 maps to chromosome 22q12-q13 (9,10).

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References:

1. Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein.  |  Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
2. Biogenesis of Tom40, core component of the TOM complex of mitochondria.  |  Rapaport, D. and Neupert, W. 1999. J Cell Biol. 146: 321-31. PMID: 10427088
3. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase.  |  van Wilpe, S., et al. 1999. Nature. 401: 485-9. PMID: 10519552
4. The TOM core complex: the general protein import pore of the outer membrane of mitochondria.  |  Ahting, U., et al. 1999. J Cell Biol. 147: 959-68. PMID: 10579717
5. The transport machinery for the import of preproteins across the outer mitochondrial membrane.  |  Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
6. Identification of mammalian TOM22 as a subunit of the preprotein translocase of the mitochondrial outer membrane.  |  Saeki, K., et al. 2000. J Biol Chem. 275: 31996-2002. PMID: 10900208
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8. Mitochondrial protein import. Tom40 plays a major role in targeting and translocation of preproteins by forming a specific binding site for the presequence.  |  Rapaport, D., et al. 1997. J Biol Chem. 272: 18725-31. PMID: 9228044
9. Functional analysis of human mitochondrial receptor Tom20 for protein import into mitochondria.  |  Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929