Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Glycolysis is an evolutionarily conserved series of ten chemical reactions that utilizes eleven enzymes to concomitantly generate pyruvate and ATP from glucose. Triosephosphate isomerase, known as TIM or TPI, is ubiquitously expressed and catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate in the glycolitic pathway. The human TIM gene spans 3.5 kilobase pairs, contains seven exons and encodes a 249 amino acid protein. The TIM promoter element contains a TATA box (positions -27 to -21) and multiple GC boxes (positions -126 to -48) that variably conform to the consensus Sp1-binding site. The GC boxes function in cis to the TATA box to control both the frequency and position of transcription initiation. Deficiencies in TIM result in a rare autosomal recessive condition where a metabolic block in glycolysis and accumulating DHAP in erythrocytes can lead to non-spherocytic hemolytic anemia, recurrent infections, cardiomyopathy, and neuromuscular dysfunctions.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using TIM (BB-7): sc-100541 for immunofluorescence, what dilution of this antibody should I use?
Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: