TCR delta抗体 (H-41) 推荐用于 human 来源的TCR δ WB, IP 和 ELISA检测

TCR δ 抗体 (H-41): sc-100289

TCR δ抗体 (H-41) is rated 4.6 out of 5 by 5.
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Datasheets

    • 抗-TCR delta 抗体 H-41 是小鼠单克隆 IgG1 (kappa轻链),TCR δ抗体,规格为100 µg/ml
    • 免疫human物种的重组TCR δ
    • 推荐用于 human 来源的TCR δ WB, IP 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取TCR δ (H-41): sc-100289免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-02-03
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Rated 5 out of 5 by from Very good TCRgd+ cell staining on FFPE After TCR gamma.20 (from Thermo Scientific) being discontinued, finally I've found in Santa Cruz Biotechnology TCR delta a solid antibody useful to recognize TCRgd cells in formalin-fixed paraffin embedded sample using immunohistochemistry. I've stained sections from tonsil and small intestine with a range of dilution from 1:25 to 1:250 and I've achieved strong and tidy stainings in all dilutions tested. Also antigen unmasking is very tolerant. It works using EDTA pH8.0 or Citrate pH 6.0 in microwave or water bath. I'm very satisfied from the results
Date published: 2020-03-12
Rated 5 out of 5 by from Excellent staining for IHC on FFPE tissues I successfully used this product for immunohistochemistry on 4 micron sections of FFPE tonsil tissues and FFPE cell block of a bone marrow blood clot. I got excellent results using 1:50-1:100 dilution for 1 hour incubation at room temperature manually or using the Ventena Benchmark Ultra autostainer. The best results were obtained using EDTA pH9 in a steamer then placed on the autostainer, or alternately using CC1 at 99 degrees C for 36 minutes. Ventena Ultraview/DAB was used for the detection system.
Date published: 2018-06-19
Rated 5 out of 5 by from Clean and Great expression of TCR Delta. I struggled for over a year to find a replacement for TCR Gamma, which was discontinued by Thermo last year. This is a great replacement for it. I can't upload a TIFF image, but the examples that I wanted to upload were from a Spleen and Colon. The retrieval for Leica Bond III was Hier2 for 40 minutes with 15 minutes of antibody incubation (modified F Protocol). Dilution that worked best was 1:200 (using Leica's diluent).
Date published: 2017-12-20
Rated 5 out of 5 by from Good for human detection Positive signal obtained at predicted molecular weight in WB for TCR delta.
Date published: 2017-02-07
Rated 3 out of 5 by from Faint band at correct MW in HeLa whole cell lysate Faint band at correct MW in HeLa whole cell lysate. -SCBT QC
Date published: 2013-12-12
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