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SRPK1 Antibody (EE-13) is a high quality monoclonal SRPK1 antibody (also designated SRPK1 antibody) suitable for the detection of the SRPK1 protein of human origin. SRPK1 Antibody (EE-13) is available as the non-conjugated anti-SRPK1 antibody. Arginine/serine-rich (RS) domain-containing proteins and their phosphorylation by specific protein kinases constitute control circuits to regulate both constitutive and alternative pre-mRNA splicing and coordinate splicing with transcription in cells. Two SR protein-specific kinases (SRPK, also designated SFRSK), SRPK1 and SRPK2, are highly specific for the phosphorylation of these RS proteins, thereby contributing to splicing regulation. SRPK1 plays a role in the condensation of sperm chromatin. SRPK2 has a stringent preference for SR dipeptides and contains a proline-rich sequence at its amino terminus. Both SRPK1 and SRPK2 are highly expresed in testis. SRPK1 is found exclusively in pancreas and SRPK2 is found exclusively in brain and lung.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Could you tell me where is the epitope binding site on SRPK1 of this antibody? Thank you.
Asked by: kristenyung
Thank you for your question. This antibody is raised against amino acids 371-470 of SRPK1 of human origin.
Answered by: Technical Support
Date published: 2019-09-11
Can sc-100443: SRPK1 (EE-13) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: Professor Griffin
Thank you for your question. Yes, sc-100443: SRPK1 (EE-13) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
Rated 5 out of
Works great in WBThis SRPK1 monoclonal antibody produces nice strong and clean WB band in IMR-32 whole cell lysate at a 1:200 dilution.
Date published: 2017-02-01
Rated 5 out of
Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human colon tissue. -SCBT QC