SRPK1 抗体 (EE-13): sc-100443

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Datasheets

- SRPK1 抗体 EE-13 是小鼠单克隆 IgG₁ κ，SRPK1抗体, 在5篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组SRPK1
- 推荐用于 human 来源的SRPK1 WB, IP, IF, IHC(P) 和 ELISA检测
- 目前，我们还没有完成SRPK1 抗体 (EE-13)的首选二抗检测试剂的鉴定。这项工作正在进行中。

SEE ALSO...

此外 SRPK 抗体包括 SRPK1和 SRPK2
ChemCruz™ Chemical SRPK1 Inhibitors可用于SRPK细胞反应的功能性分析

SRPK1 Antibody (EE-13) - Western Blotting - Image 33816

SRPK1 Antibody (EE-13) - Immunohistochemistry - Image 36025

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SRPK1 Antibody (EE-13) is an IgG₁ κ mouse monoclonal SRPK1 antibody (also designated SRPK1 antibody) that detects the SRPK1 protein of human origin by WB, IP, IF, IHC(P) and ELISA. SRPK1 Antibody (EE-13) is available as the non-conjugated anti-SRPK1 antibody. Arginine/serine-rich (RS) domain-containing proteins and their phosphorylation by specific protein kinases constitute control circuits to regulate both constitutive and alternative pre-mRNA splicing and coordinate splicing with transcription in cells. Two SR protein-specific kinases (SRPK, also designated SFRSK), SRPK1 and SRPK2, are highly specific for the phosphorylation of these RS proteins, thereby contributing to splicing regulation. SRPK1 plays a role in the condensation of sperm chromatin. SRPK2 has a stringent preference for SR dipeptides and contains a proline-rich sequence at its amino terminus. Both SRPK1 and SRPK2 are highly expresed in testis. SRPK1 is found exclusively in pancreas and SRPK2 is found exclusively in brain and lung.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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References:

Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes. | Wang, HY., et al. 1999. Genomics. 57: 310-5. PMID: 10198174
SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1. | Papoutsopoulou, S., et al. 1999. Nucleic Acids Res. 27: 2972-80. PMID: 10390541
Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1. | Tang, Z., et al. 2000. Mol Cell Biol. 20: 816-24. PMID: 10629038
Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles. | Kuroyanagi, N., et al. 1998. Biochem Biophys Res Commun. 242: 357-64. PMID: 9446799
SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. | Wang, HY., et al. 1998. J Cell Biol. 140: 737-50. PMID: 9472028

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	SRPK1	Entrez Gene ID: 6732	6p21.31	NM_003137	UniProt ID: Q96SB4	601939

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Protocols Online

優選的 SRPK 抗體是 SRPK 抗體 (D-7): sc-398432.

Could you tell me where is the epitope binding site on SRPK1 of this antibody? Thank you.

Asked by: kristenyung

Thank you for your question. This antibody is raised against amino acids 371-470 of SRPK1 of human origin.

Answered by: Technical Support

Date published: 2019-09-11

Can sc-100443: SRPK1 (EE-13) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin

Thank you for your question. Yes, sc-100443: SRPK1 (EE-13) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 5 out of 5 by Germaine from Works great in WB This SRPK1 monoclonal antibody produces nice strong and clean WB band in IMR-32 whole cell lysate at a 1:200 dilution.

Date published: 2017-02-01

Rated 5 out of 5 by MC from Produced exceptional immunoperoxidase nuclear Produced exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human colon tissue. -SCBT QC

Date published: 2015-03-15

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