SPT3 抗体 (71-S): sc-101157

SPT3 Antibody (71-S) is an IgM mouse monoclonal SPT3 antibody that detects SPT3 of human origin by WB, IP and ELISA. SPT3 Antibody (71-S) is available as the non-conjugated anti-SPT3 antibody form. The Saccharomyces cerevisiae SAGA complex is a multifunctional coactivator that regulates transcription by RNA polymerase II. In yeast, SPT3 is a component of the multiprotein SPT-ADA-GCN5 acetyltransferase (SAGA) complex that integrates proteins with transcription coactivator/adaptor functions, histone acetyltransferase activity, and core promoter-selective functions involving interactions with the TATA-binding protein. The human STAGA complex contains homologs of most yeast SAGA components. STAGA has acetyl coenzyme A-dependent transcriptional coactivator functions from a chromatin-assembled template in vitro and associates in HeLa cells with spliceosome-associated proteins. Amino acid sequence comparisons between human SPT3 and its counterparts in yeast reveal three highly conserved domains, with the most conserved 92-amino acid N-terminal domain being 25% identical with human TAFII18.

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References:

1. Inhibition of TATA-binding protein function by SAGA subunits Spt3 and Spt8 at Gcn4-activated promoters.  |  Belotserkovskaya, R., et al. 2000. Mol Cell Biol. 20: 634-47. PMID: 10611242
2. Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo.  |  Martinez, E., et al. 2001. Mol Cell Biol. 21: 6782-95. PMID: 11564863
3. Spt3 and Mot1 cooperate in nucleosome remodeling independently of TBP recruitment.  |  Topalidou, I., et al. 2004. EMBO J. 23: 1943-8. PMID: 15057269
4. Molecular architecture of the S. cerevisiae SAGA complex.  |  Wu, PY., et al. 2004. Mol Cell. 15: 199-208. PMID: 15260971
5. The NOT, SPT3, and MOT1 genes functionally interact to regulate transcription at core promoters.  |  Collart, MA. 1996. Mol Cell Biol. 16: 6668-76. PMID: 8943321
6. Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family.  |  Birck, C., et al. 1998. Cell. 94: 239-49. PMID: 9695952
7. A human SPT3-TAFII31-GCN5-L acetylase complex distinct from transcription factor IID.  |  Martinez, E., et al. 1998. J Biol Chem. 273: 23781-5. PMID: 9726987
8. Characterization of a human homologue of the Saccharomyces cerevisiae transcription factor spt3 (SUPT3H).  |  Yu, J., et al. 1998. Genomics. 53: 90-6. PMID: 9787080