Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
SPRED1 (sprouty-related, EVH1 domain containing 1), also known as NFLS, is a 444 amino acid protein that contains one KBD domain, one sprouty domain and one WH1 domain. Localized to the cell membrane and to cholesterol-rich membrane raft fractions, SPRED1 functions as a tyrosine kinase that regulates the activity of the ERK (also known as MAP kinase) cascade by inhibiting the growth-factor-mediated activation of ERK. SPRED1 can act independently as a homodimer or can function as a heterodimer with SPRED2 and, in addition to its ability to regulate ERK, is thought to negatively regulate the development of blood cells in bone marrow. Defects in the gene encoding SPRED1 are the cause of neurofibromatosis type 1-like syndrome (NFLS), an autosomal dominant disease that is characterized by multiple cafe-au-lait spots, axillary freckling and macrocephaly.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended fixation method for immunofluorescence staining with SPRED1 (M23-P2G3): sc-101392 monoclonal antibody?
Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: