SPF30 抗体 (SQ-16): sc-100338

4.0(2)

写一个评价提问

Datasheets

- SPF30抗体(SQ-16)是小鼠单克隆mouse IgG₁ κ (kappa轻链)，规格为50 µg/0.5 ml
- 免疫human物种的重组SPF30
- 推荐用于 mouse, rat 和 human 来源的SPF30 WB, IP, IF, IHC(P) 和 ELISA检测
- 目前，我们还没有完成SPF30 抗体 (SQ-16)的首选二抗检测试剂的鉴定。这项工作正在进行中。

SEE ALSO...

Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

SPF30 Antibody (SQ-16) - Western Blotting - Image 33714

SPF30 Antibody (SQ-16) - Immunohistochemistry - Image 35976

SPF30 Antibody (SQ-16) - Western Blotting - Image 20174

点击图片或放大按钮来放大

SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (Survival Motor Neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	SMNDC1	Entrez Gene ID: 10285	10q25.2	XM_005269382, NM_005871	UniProt ID: O75940	603519

Download Datasheet

Protocols Online

優選的 SPF30 抗體是 SPF30 抗體 (G-8): sc-376887.

Can sc-100338: SPF30 (SQ-16) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt

Thank you for your question. Yes, sc-100338: SPF30 (SQ-16) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 4 out of 5 by NZ from Great immunoperoxidase nuclear staining in Great immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human testis tissue. -SCBT QC

Date published: 2015-03-12

选择店铺

选择语言

选择币种

SPF30 抗体 (SQ-16): sc-100338

QUICK LINKS

SEE ALSO...

優選的 SPF30 抗體是 SPF30 抗體 (G-8): sc-376887.

Can sc-100338: SPF30 (SQ-16) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?