SOD-1抗体 (24) 推荐用于 mouse, rat 和 human 来源的SOD-1 WB, IP, IF, IHC(P) 和 ELISA检测

SOD-1 抗体 (24): sc-101523

SOD-1抗体 (24) is rated 4.3 out of 5 by 11.
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Datasheets

    • SOD-1抗体(24)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
    • raised against full-length recombinant SOD-1 of human origin
    • 抗-SOD-1 抗体 (24) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 SOD-1
    • 抗-SOD-1 抗体 (24) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取SOD-1 (24): sc-101523免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

I am using SOD-1 (24): sc-101523 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2018-11-15
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Rated 5 out of 5 by from super strong Antibody detects single extremely strong band at around 17kDa in U2OS lysates in WB. Dilution 1:2000. I would recommend higher dilution, like 1:3000-4000
Date published: 2019-10-22
Rated 1 out of 5 by from No nos ha funcionado. Probado en lisados de celulas AR42J, no hemosd etectado ninguna banda.
Date published: 2019-05-09
Rated 5 out of 5 by from Good results Used in PMS from primary neuronal culture lysate, reallly good and reproducible results.
Date published: 2019-03-12
Rated 5 out of 5 by from Very good in WB Application: Western blotting Dilution 1:1000 Cell lysate of C2C12 Very strong and specific band
Date published: 2018-10-05
Rated 4 out of 5 by from good results in WB Good results in Western Blot, we used it 1:500 in milk
Date published: 2018-05-16
Rated 5 out of 5 by from 效果不错 一个月前买的这个产品,效果很好,下次继续买这家的。我还会推荐别人买,好东西大家都支持,圣克鲁斯加油!
Date published: 2018-04-04
Rated 4 out of 5 by from the only SOD1 that worked tested SOD1 monoclonal ab (sc-101523 (1:1000), sc-515404 (1:100) and sc-271014 (1:100)) in western blots with 100 ug protein lysates from rat heart or rat artery transferred onto nitrocellulose membranes probed with LiCor secondary abs. only SOD1: sc-101523 produced a distinct band at expected kD.
Date published: 2017-11-17
Rated 5 out of 5 by from Nice SOD1 detection in Jurkat cells We used this antibody for expression detection of SOD1 by western blot. We got a nice band around the correct molecular weight in Jurkat cells.
Date published: 2017-02-07
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