Smurf1抗体 (45-K) 推荐用于 mouse, rat 和 human 来源的Smurf1 WB, IP, IF, IHC(P) 和 ELISA检测

Smurf1 抗体 (45-K): sc-100616

Smurf1抗体 (45-K) is rated 4.6 out of 5 by 14.
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Datasheets

    • 抗-Smurf1 抗体 45-K 是小鼠单克隆 IgG2a (kappa轻链),Smurf1抗体,规格为100 µg/ml
    • raised against an internal portion of Smurf1 of human origin
    • 推荐用于 mouse, rat 和 human 来源的Smurf1 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Smurf1 (45-K): sc-100616免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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Can Smurf1 (45-K): sc-100616 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Anna99
Thank you for your question. Yes, Smurf1 (45-K): sc-100616 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 5
Date published: 2017-01-19
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Rated 5 out of 5 by from Excellent for detecting SMURF1 Utilized this trial antibody on mouse lysates (10ug) in a 1:200 dilution on a western blot. Gave a very clean and strong band.
Date published: 2020-03-11
Rated 4 out of 5 by from 能够显出来条带 之前买的H-60,显人源蛋白,有很多杂带,效果不怎么好,后来换成45-K,效果还是挺好的,只是做小鼠蛋白WB时,感觉效果不怎么好
Date published: 2018-10-19
Rated 4 out of 5 by from works well in WB detects a decent band of the right size, but there are some weak unspecific bands as well.
Date published: 2018-05-17
Rated 5 out of 5 by from It's specific I got a free sample from SC, and this antibody is specific for SMURF1 protein.
Date published: 2018-04-12
Rated 5 out of 5 by from quite good for wb 1:500 diluted for wb of rat protein ,the band is strong and special!
Date published: 2017-11-05
Rated 1 out of 5 by from terible cross-reactivity with unknown proteins There was a very faint band at the MW expected for this protein, but a very dark band at 26kD and multiple other bands. I would not recommend this antibody.
Date published: 2017-10-23
Rated 5 out of 5 by from Good I recept it days ago and used it recently. To my delight, the product was so good!
Date published: 2017-09-07
Rated 5 out of 5 by from 这个抗体好用 两个月前申请试用装,在皮肤中进行免疫荧光实验检测,做出了阳性结果,还没有重复,希望能对大家有所帮助。
Date published: 2017-08-24
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