Smad7抗体 (Z8-B) 推荐用于 mouse, rat 和 human 来源的Smad7 WB, IP, IF, IHC(P) 和 ELISA检测

Smad7 抗体 (Z8-B): sc-101152

Smad7抗体 (Z8-B) is rated 4.1 out of 5 by 7.
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Datasheets

    • Smad7抗体(Z8-B)是小鼠单克隆IgG2a (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组Smad7
    • 推荐用于 mouse, rat 和 human 来源的Smad7 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 看 Smad7 (B-8): sc-365846,可查看有标记的 Smad7 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 关于如何获取Smad7 (Z8-B): sc-101152免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

I would like to know what the second blot line mean in the picture of WB(Smad7 抗体 (Z8-B): sc-101152). My result have two blot lines, I can't find the answer in the introduction. thank you a lot.

Asked by: house
Thank you for your question. We have not characterized both bands which were observed in HeLa whole cell lysate when Western blotting with this antibody. The predicted molecular weight of Smad7 is 46 kDa, and other isoforms of similar size have been reported according to Uniprot. https://www.uniprot.org/uniprot/O15105#sequences
Answered by: Technical Support
Date published: 2018-12-14

I would like to use Smad7 Antibody (Z8-B): sc-101152 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-101152 is not available directly conjugated, but we have an alternative Smad7 antibody (sc-365846) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 6
Date published: 2017-01-11

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Smad7 Antibody (Z8-B): sc-101152?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-11

您好!smad7 (B-8):sc-365846 smad7(28-B):sc-101152 可以领两个免费小样吗?

Asked by: hf123q
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Rated 3 out of 5 by from Works in chicken This antibody works by Western blot on chicken embryo lysates at a dilution of 1:200.
Date published: 2018-03-26
Rated 5 out of 5 by from Works well in my Immunofluorescence I tried 1:50 in my fibroblasts IF, got strong/reasonable staining. I have not yet tried Western Blot.
Date published: 2017-12-22
Rated 5 out of 5 by from Strong WB and IHC ! Very nice product for IHC studies; also clean western
Date published: 2017-01-15
Rated 4 out of 5 by from Works in IHC(P) Positive staining seen in IHC with paraffin-embedded human tissue samples.
Date published: 2016-12-23
Rated 3 out of 5 by from Showed nuclear staining of varying strength Showed nuclear staining of varying strength in human placenta tissue (FFPE) -SCBT QC
Date published: 2015-02-10
Rated 5 out of 5 by from Excellent detection of Smad7 in human corneal Excellent detection of Smad7 in human corneal fibroblasts -SCBT Publication Review
Date published: 2013-07-02
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