Smad5抗体 (YY-6) 推荐用于 mouse, rat 和 human 来源的Smad5 WB, IP, IF 和 ELISA检测

Smad5 抗体 (YY-6): sc-101151

Smad5抗体 (YY-6) is rated 4.0 out of 5 by 6.
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Datasheets

    • 抗-Smad5 抗体 YY-6 是小鼠单克隆 IgG2a κ,Smad5抗体, 在4篇文献中引用,规格为100 µg/ml
    • 免疫human物种的重组Smad5
    • 推荐用于 mouse, rat 和 human 来源的Smad5 WB, IP, IF 和 ELISA检测
    • 关于如何获取Smad5 (YY-6): sc-101151免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 Smad5 Antibody (YY-6) for WB 的首选应用二抗。这些试剂现在以套装形式提供Smad5 Antibody (YY-6)。 (见下方订购信息).
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I am using Smad5 (YY-6): sc-101151 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 1 out of 5 by from It does not work on mouse liver protein extracts I receive the trial size and I tried WB analysis on liver protein extracts from mice but in our hand it did not work. It did not recognize the total SMAD5 protein.
Date published: 2021-01-20
Rated 4 out of 5 by from Works in chicken This antibody works by Western blot on chicken embryo lysates at a dilution of 1:200.
Date published: 2018-03-26
Rated 4 out of 5 by from working well it work well and located clear,and just follow the normal protocal
Date published: 2018-02-09
Rated 5 out of 5 by from great antibody It works great for WB at 1:5,000 dilution. lt locates in the nuclear.
Date published: 2017-11-14
Rated 5 out of 5 by from Great IF images with nuclear localization Great IF images with nuclear localization in HeLa cells. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Good specificity and signal strength for Good specificity and signal strength for WB in HeLa nuclear extract. -SCBT QC
Date published: 2014-04-26
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