Smad3抗体 (38-Q) 推荐用于 mouse, rat 和 human 来源的Smad3 WB, IP, IF, IHC(P) 和 ELISA检测

Smad3 抗体 (38-Q): sc-101154

Smad3抗体 (38-Q) is rated 4.7 out of 5 by 11.
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Datasheets

    • Smad3抗体(38-Q)是小鼠单克隆IgG2a (kappa轻链),规格为50 µg/0.5 ml
    • 免疫human物种的重组Smad3
    • 推荐用于 mouse, rat 和 human 来源的Smad3 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 看 Smad2/3 (C-8): sc-133098,可查看有标记的 Smad3 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 关于如何获取Smad3 (38-Q): sc-101154免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 5 out of 5 by from my review for Smad3 (38-Q, sc-101154) antibody I tested a free antibody for Smad3 (38-Q, sc-101154) antibody using mouse fibrotic kidney samples for Western blot. We loaded 80 ug proteins in each slot in the SDS gel. The antibody was diluted in 1:500 in 3% BSA/PBST and incubated overnight with the blot. We can observe a very clear band around 55 kd. We have repeated this experiment several times and used the fresh diluted antibody on the fresh blot.
Date published: 2018-05-26
Rated 5 out of 5 by from 抗体很好 之前用的好多家都不好,因为样品浓度低,这个抗体第一次就出条带了,只能说抗体特异性好,之前都打不开网页,所以到现在才评论。
Date published: 2018-04-28
Rated 4 out of 5 by from Good for WB 1:100 diluted for wb of rat protein ,the band is clear and special
Date published: 2018-01-24
Rated 5 out of 5 by from works really well This antibody has been tested on the Infrared detection system. It works really well and I strongly recommend
Date published: 2017-02-06
Rated 5 out of 5 by from Well published Smad3 (38-Q) monoclonal antibody is well published for Western blot. Highly recommended!
Date published: 2016-12-20
Rated 5 out of 5 by from good results in WB I've obtained good results in WB, setting the dilution to 1:100.
Date published: 2016-11-24
Rated 5 out of 5 by from Fresh out of the antibody sample Fresh out of the antibody sample, the experimental results very well
Date published: 2016-08-08
Rated 5 out of 5 by from This antibody has worked well in WB This antibody has worked well in WB.
Date published: 2015-10-16
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