Smad2抗体 (YZ-13) 推荐用于 mouse, rat 和 human 来源的Smad2 WB, IP, IF, IHC(P) 和 ELISA检测

Smad2 抗体 (YZ-13): sc-101153

Smad2抗体 (YZ-13) is rated 4.6 out of 5 by 7.
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Datasheets

    • Smad2抗体(YZ-13)是小鼠单克隆IgG2a (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组Smad2
    • 推荐用于 mouse, rat 和 human 来源的Smad2 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 看 Smad2/3 (C-8): sc-133098,可查看有标记的 Smad2 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 关于如何获取Smad2 (YZ-13): sc-101153免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Can this antibody be used for detected phosphorylated smad2 via IF in free floating coronal brain sections? Or do you have another antibody for that? Kindly let me know.

Asked by: Sonam
Thank you for your question. Our Smad2 (YZ-13) sc-101153 antibody is appropriate for detection total Smad2, phosphorylated and non-phosphorylated, in IF. We have not specifically validated it for free floating sections. We do not currently have a p-Smad2 specific antibody. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-07-18

Can Smad2 (YZ-13): sc-101153 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Smad2 (YZ-13): sc-101153 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 5 out of 5 by from Good antibody! 1:200 diluted for wb of rat heart protein,the band is strong and special!
Date published: 2018-01-01
Rated 5 out of 5 by from Solid antibody Smad2 (YZ-13) works great for WB, IHC(P), and IF. Well cited in literature for WB.
Date published: 2017-01-20
Rated 5 out of 5 by from Happy to use it The background is clean and the staining is good. Happy to buy it.
Date published: 2016-11-24
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HeLa. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Good nuclear staining and cytoplasmic localization Good nuclear staining and cytoplasmic localization with paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-03-30
Rated 3 out of 5 by from Good for WB analyzing Smad2 in HeLa nuclear Good for WB analyzing Smad2 in HeLa nuclear extract. Some background bands detected -SCBT QC
Date published: 2015-03-29
Rated 4 out of 5 by from Worked well with transfected 293T whole cell Worked well with transfected 293T whole cell lysate by WB. -SCBT QC
Date published: 2013-12-03
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