Set1 抗体 (1.4): sc-101858

Set1 Antibody (1.4) is an IgG₁ κ mouse monoclonal Set1 antibody (also designated Set1 antibody) that detects the Set1 protein of Saccharomyces cerevisiae origin by WB and IP. Set1 Antibody (1.4) is available as both the non-conjugated anti-Set1 antibody form, as well as multiple conjugated forms of anti-Set1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The trithorax gene family functions to maintain specific patterns of gene expression throughout cellular development by activating or repressing transcription. Members of this family contain a highly conserved 130- to 140-amino acid motif designated the SET domain, and include the Drosophila and human trithorax and Su(var)3-9 protein homologues. The Saccharyomyces cerevisiae trithorax homolog Set1, a histone H3-lysine 4 (H3-K4) methylase, regulates chromatin structure, DNA repair, and telomeric function. It also regulates rDNA silencing, either directly or indirectly, via H3 methylation. Set1 forms a complex with six other nonessential proteins. This complex, designated COMPASS (Complex Proteins Associated with Set1) includes Cps25, Cps30, Cps35, Cps40, Cps50, and Cps60. Phosphorylation of the C-terminal domain (CTD) of Pol II at serine 5 by the TFIIH-associated kinase mediates the transition between initiation and elongation. Set1 associates with this phosphorylated form of Poll II, and may provide a molecular memory of recent transcriptional activity.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The set1Delta mutation unveils a novel signaling pathway relayed by the Rad53-dependent hyperphosphorylation of replication protein A that leads to transcriptional activation of repair genes.  |  Schramke, V., et al. 2001. Genes Dev. 15: 1845-58. PMID: 11459833
2. COMPASS: a complex of proteins associated with a trithorax-related SET domain protein.  |  Miller, T., et al. 2001. Proc Natl Acad Sci U S A. 98: 12902-7. PMID: 11687631
3. Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.  |  Briggs, SD., et al. 2001. Genes Dev. 15: 3286-95. PMID: 11751634
4. A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3.  |  Nagy, PL., et al. 2002. Proc Natl Acad Sci U S A. 99: 90-4. PMID: 11752412
5. COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression.  |  Krogan, NJ., et al. 2002. J Biol Chem. 277: 10753-5. PMID: 11805083
6. Evidence that Set1, a factor required for methylation of histone H3, regulates rDNA silencing in S. cerevisiae by a Sir2-independent mechanism.  |  Bryk, M., et al. 2002. Curr Biol. 12: 165-70. PMID: 11818070
7. Targeted recruitment of Set1 histone methylase by elongating Pol II provides a localized mark and memory of recent transcriptional activity.  |  Ng, HH., et al. 2003. Mol Cell. 11: 709-19. PMID: 12667453
8. SET1, a yeast member of the trithorax family, functions in transcriptional silencing and diverse cellular processes.  |  Nislow, C., et al. 1997. Mol Biol Cell. 8: 2421-36. PMID: 9398665