SerpinB8抗体 (PI-8) 推荐用于 human 来源的SerpinB8 WB, IP, IF, IHC(P) 和 ELISA检测

SerpinB8 抗体 (PI-8): sc-101371

SerpinB8抗体 (PI-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • 抗-SerpinB8 抗体 PI-8 是小鼠单克隆 IgG1 (kappa轻链),SerpinB8抗体,规格为200 µg/ml
    • 免疫human物种的SerpinB8
    • SerpinB8 抗体 (PI-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 SerpinB8
    • 抗-SerpinB8 抗体 (PI-8) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取SerpinB8 (PI-8): sc-101371免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Can SerpinB8 (PI-8): sc-101371 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, SerpinB8 (PI-8): sc-101371 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Westernblot Good quality Mab.Used it in WB,tried dilution 1:200 and 1:500, bot strong signal for human colon cell lines detection.
Date published: 2018-01-27
Rated 5 out of 5 by from Used for western blot analysis of SerpinB8 Used for western blot analysis of SerpinB8 expression in mucosa oropharynx, spleen, placenta, pituitary glad, epidermis, stomach, colon, lung, kidney, liver, heart, brain and pancreas human tissue lysates. Reproduced, with permission, from Strik MC, Bladergroen BA, Wouters D, Kisiel W, Hooijberg JH, Verlaan AR, Hordijk PL, Schneider P, Hack CE, Kummer JA: Distribution of the human intracellular serpin protease inhibitor 8 in human tissues. Journal of Histochemistry & Cytochemistry. 50(11):1443-54, 2002. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclear Produced beautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Works well for western blotting in human Works well for western blotting in human SerpinB8 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-06
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