S-100A14 抗体 (5): sc-101506

3.0(2)

- S-100A14 抗体 5 是小鼠单克隆 IgG₁ (kappa轻链)，S-100A14抗体，规格为200 µg/ml
- 免疫human物种的重组S-100A14
- S-100A14 抗体 (5) 推荐用于IF, IHC(P) 和 ELISA，检测human 来源的 S-100A14
- 抗-S-100A14 抗体 (5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取S-100A14 (5): sc-101506的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 S-100A14 Antibody (5) for IHC(P) 首选应用二抗. 这些试剂现在以套形式提供 S-100A14 Antibody (5) (见下方订购信息).

SEE ALSO...

此外 S-100 抗体包括 S-100 α chain, S-100 β chain, S-100A10, S-100A13, S-100A14, S-100A16, S-100A2, S-100A3 和 S-100P
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

S-100A14 Antibody (5) is an IgG₁ κ mouse monoclonal S-100A14 antibody (also designated S100 calcium binding protein A14 antibody, EF-hand type helix-loop-helix structural domain-containing, calcium (Ca2+)-binding protein antibody, Damage-associated molecular patterns (DAMPs) related antibody, or noninfectious inflammatory response protein specific antibody) suitable for the detection of the S-100A14 protein of human origin by IF, IHC(P) and ELISA. S-100A14 Antibody (5) is available as both the non-conjugated anti-S-100A14 antibody form, as well as multiple conjugated forms of anti-S-100A14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The S-100 protein family consists of a group of calcium-binding proteins, which exhibit cell and tissue-specific expression. The expression levels of its members differ in various pathological conditions. The extracellular functions of the S-100 family may include the ability to enhance neurite outgrowth, involvement in inflammation, and motility of tumor cells. S100 calcium binding protein A14 (S-100A14), a member of the S-100 protein family, is expressed highest in the colon and moderately in the thymus, kidney, liver, small intestine, and lung. Low expression of S-100A14 is observed in the heart, while no detection is seen in brain, skeletal muscle, spleen, placenta and peripheral blood leukocytes. S-100A14 is a 104-amino acid protein that is 68% homologous to the S-100A13 protein. It contains 2 EF-hand Ca2+-binding domains, a myristoylation motif, a glycosylation site, and several potential protein kinase phosphorylation sites.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	S100A14	Entrez Gene ID: 57402	1q21.3	XM_005245362, XM_017001875, NM_020672	UniProt ID: Q9HCY8	607986

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗S-100A14抗体(5)在各种科学出版物中有0篇引文。

针对哺乳动物靶标蛋白的一抗，如抗S-100A14 抗体 (5)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

S-100A14 抗体 (5) Specifications

替代产品名称

S100 protein A14 (S100A14)

产品克隆名称

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

抗原表位靶标

S-100A14

抗原表位种属

human

产品反应性/检测

S-100A14

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

IF, IHC(P), ELISA

瓶的信息

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

0篇各种科学出版物的引文

可选的偶联物/形式

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗S-100A14抗体(5)推荐用于以下应用：IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

What is the recommended secondary reagent to be used with sc-101506: S-100A14 (5) mouse monoclonal primary?

Asked by: Germaine

We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Technical Support

Date published: 2017-04-12

Rated 4 out of 5 by Rimak from S-100A14 Antibody I used this antibody in A549 lysates and also in primary cells for western blotting.I got very strong bands.

Date published: 2016-12-11

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