S-100A13抗体 (63-Y) 推荐用于 human 来源的S-100A13 WB, IP, IF 和 ELISA检测

S-100A13 抗体 (63-Y): sc-100935

S-100A13抗体 (63-Y) is rated 4.5 out of 5 by 4.
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Datasheets

    • 抗-S-100A13 抗体 63-Y 是小鼠单克隆 IgG2a (kappa轻链),S-100A13抗体,规格为100 µg/ml
    • 免疫human物种的重组S-100A13
    • 推荐用于 human 来源的S-100A13 WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取S-100A13 (63-Y): sc-100935免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

I am using S-100A13 (63-Y): sc-100935 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Very good. Super strong signal but around 80kda using Hela cells lysate. Used 1-200 dilution in blocking solution 3% BSA 2% milk overnight 4C. PVDF membrane.
Date published: 2019-08-23
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear staining Beautiful immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-28
Rated 4 out of 5 by from Strong crisp band seen in WB using MCF7 whole Strong crisp band seen in WB using MCF7 whole cell lysate. -SCBT QC
Date published: 2015-03-10
Rated 4 out of 5 by from Nice bands detected in WB using HUV Nice bands detected in WB using HUV-EC-C and ECV304 whole cell lysates. -SCBT QC
Date published: 2015-01-06
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