Rotavirus capsid抗体 (2B4) 推荐用于 Rotavirus 来源的inner capsid protein VP6 WB, IF, IHC(P) 和 ELISA检测

Rotavirus capsid 抗体 (2B4): sc-101363

Rotavirus capsid抗体 (2B4) is rated 4.5 out of 5 by 2.
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Datasheets

    • Rotavirus capsid抗体(2B4)是小鼠单克隆IgG2b κ, 在6篇文献中引用,规格为50 µg/0.5 ml
    • raised against recombinant Rotavirus capsid
    • 推荐用于 Rotavirus 来源的inner capsid protein VP6 WB, IF, IHC(P) 和 ELISA检测
    • 关于如何获取Rotavirus capsid (2B4): sc-101363免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 目前,我们还没有完成Rotavirus capsid 抗体 (2B4)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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Hello, I´m not sure how to store this antibody. Datasheet says -20 °C and the vial says Store at 4°C. Which one is correct?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I suppose this Ab works only for rotavirus group A. Does it reacts against porcine rotavirus A as well? Are there possible crossreactions to rotavirus group B and C?

Asked by: faun365
Thank you for your question. You can find information on the reactivity of Rotavirus capsid Antibody (2B4): sc-101363 in porcine tissue in PMID 30249258. For further information please contact technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Hello, I write to ask an information about this antibody we bought recently... I have to performed an IHC-(P) on intestinal tissues...What kind of proteolitic pretratment is more suitable to unmask VP6 rotavirus protein? Trypsin? Thank you in advance! 

Asked by: Chiara71
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval when using this antibody on formalin-fixed paraffin-embedded tissue sections. A link to our recommended protocol on our website is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-07-19

Can sc-101363: Rotavirus capsid (2B4) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-101363: Rotavirus capsid (2B4) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Reliable Antibody for WB Works well in WB, strong clear signal with no background.
Date published: 2017-01-25
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