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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
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Rotaviruses, a genus of the family Reoviridae, are double-stranded RNA viruses that are one of the leading causes of gastroenteritis in infants and young children. The virus exists as seven species, designated Rotavirus A, B, C, D, E, F and G, with Rotavirus A being the most common cause of infection. Rotavirus is transmitted via the fecal-oral route and, once in the body, infects cells of the small intestine, producing an enterotoxin that alters the permeability of the cell wall, causing severe diarrhea and dehydration. The entry of Rotavirus into cells occurs by either direct penetration of the cell membrane or endocytosis followed by membrane vesicle solubilization, both of which are facilitated by the Rotavirus capsid. The Rotavirus capsid is composed of three concentric protein layers, the outer two of which are called VP4 and VP7 and are sacrificed in a calcium-dependent manner during viral entry into the cell.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I suppose this Ab works only for rotavirus group A. Does it reacts against porcine rotavirus A as well? Are there possible crossreactions to rotavirus group B and C?
Asked by: faun365
Thank you for your question. You can find information on the reactivity of Rotavirus capsid Antibody (2B4): sc-101363 in porcine tissue in PMID 30249258.
For further information please contact technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
Hello, I write to ask an information about this antibody we bought recently... I have to performed an IHC-(P) on intestinal tissues...What kind of proteolitic pretratment is more suitable to unmask VP6 rotavirus protein? Trypsin? Thank you in advance!
Asked by: Chiara71
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval when using this antibody on formalin-fixed paraffin-embedded tissue sections. A link to our recommended protocol on our website is provided below as a reference.
Answered by: Technical Support
Date published: 2017-07-19
Can sc-101363: Rotavirus capsid (2B4) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: Cweed
Thank you for your question. Yes, sc-101363: Rotavirus capsid (2B4) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: