Rotavirus capsid 抗体 (2B4): sc-101363

4.5(2)

写一个评价提问

Datasheets

- Rotavirus capsid抗体(2B4)是小鼠单克隆IgG_2b κ, 在10篇文献中引用，规格为50 µg/0.5 ml
- raised against recombinant Rotavirus capsid
- 推荐用于 Rotavirus 来源的inner capsid protein VP6 WB, IF, IHC(P) 和 ELISA检测
- 关于如何获取Rotavirus capsid (2B4): sc-101363的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成Rotavirus capsid 抗体 (2B4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

References:

Rotavirus capsid protein VP5* permeabilizes membranes. | Denisova, E., et al. 1999. J Virol. 73: 3147-53. PMID: 10074166
Antibodies to rotavirus outer capsid glycoprotein VP7 neutralize infectivity by inhibiting virion decapsidation. | Ludert, JE., et al. 2002. J Virol. 76: 6643-51. PMID: 12050377
Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice. | Yu, J. and Langridge, W. 2003. Transgenic Res. 12: 163-9. PMID: 12739884
Trypsin is associated with the rotavirus capsid and is activated by solubilization of outer capsid proteins. | Benureau, Y., et al. 2005. J Gen Virol. 86: 3143-3151. PMID: 16227238
Rotavirus anti-VP6 secretory immunoglobulin A contributes to protection via intracellular neutralization but not via immune exclusion. | Corthεsy, B., et al. 2006. J Virol. 80: 10692-9. PMID: 16956954
Assembly of highly infectious rotavirus particles recoated with recombinant outer capsid proteins. | Trask, SD. and Dormitzer, PR. 2006. J Virol. 80: 11293-304. PMID: 16971442
Intracellular disassembly of infectious rotavirus particles by depletion of Ca2+ sequestered in the endoplasmic reticulum at the end of virus cycle. | Ruiz, MC., et al. 2007. Virus Res. 130: 140-50. PMID: 17643542
Rotavirus infection induces the phosphorylation of eIF2alpha but prevents the formation of stress granules. | Montero, H., et al. 2008. J Virol. 82: 1496-504. PMID: 18032499
Geometric mismatches within the concentric layers of rotavirus particles: a potential regulatory switch of viral particle transcription activity. | Libersou, S., et al. 2008. J Virol. 82: 2844-52. PMID: 18184711

Download Datasheet

Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗Rotavirus capsid抗体(2B4)在各种科学出版物中有10篇引文。

针对哺乳动物靶标蛋白的一抗，如抗Rotavirus capsid 抗体 (2B4)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

Rotavirus capsid 抗体 (2B4) Specifications

替代产品名称

Rotavirus capsid

产品克隆名称

2B4

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2b kappa light chain

氨基酸

抗原表位靶点

Rotavirus capsid

抗原表位种属

产品反应性/检测

inner capsid protein VP6

种属反应性/检测

Rotavirus

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IF, IHC(P), ELISA

瓶的信息

Each vial contains 50 µg IgG_2b kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

10篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗，如抗Rotavirus capsid抗体 (2B4)，在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼，非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗Rotavirus capsid抗体推荐用于以下应用：WB (Western blotting), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和 TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Hello, I´m not sure how to store this antibody. Datasheet says -20 °C and the vial says Store at 4°C. Which one is correct?

Asked by: Anonymous

Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.

Answered by: Technical Support

Date published: 2022-09-22

I suppose this Ab works only for rotavirus group A. Does it reacts against porcine rotavirus A as well? Are there possible crossreactions to rotavirus group B and C?

Asked by: faun365

Thank you for your question. You can find information on the reactivity of Rotavirus capsid Antibody (2B4): sc-101363 in porcine tissue in PMID 30249258. For further information please contact technical service.

Answered by: SCBT Heidelberg Support

Date published: 2019-01-06

Hello, I write to ask an information about this antibody we bought recently... I have to performed an IHC-(P) on intestinal tissues...What kind of proteolitic pretratment is more suitable to unmask VP6 rotavirus protein? Trypsin? Thank you in advance! 

Asked by: Chiara71

Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval when using this antibody on formalin-fixed paraffin-embedded tissue sections. A link to our recommended protocol on our website is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-07-19

Can sc-101363: Rotavirus capsid (2B4) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed

Thank you for your question. Yes, sc-101363: Rotavirus capsid (2B4) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 5 out of 5 by DefinitelyNotMatt from Reliable Antibody for WB Works well in WB, strong clear signal with no background.

Date published: 2017-01-25

选择店铺

选择语言

选择币种

Rotavirus capsid 抗体 (2B4): sc-101363

QUICK LINKS

SEE ALSO...

References:

Rotavirus capsid 抗体 (2B4) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶点

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

Hello, I´m not sure how to store this antibody. Datasheet says -20 °C and the vial says Store at 4°C. Which one is correct?

I suppose this Ab works only for rotavirus group A. Does it reacts against porcine rotavirus A as well? Are there possible crossreactions to rotavirus group B and C?

Hello, I write to ask an information about this antibody we bought recently... I have to performed an IHC-(P) on intestinal tissues...What kind of proteolitic pretratment is more suitable to unmask VP6 rotavirus protein? Trypsin? Thank you in advance!

Can sc-101363: Rotavirus capsid (2B4) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Rotavirus capsid 抗体 (2B4): sc-101363

QUICK LINKS

SEE ALSO...

References:

Rotavirus capsid 抗体 (2B4) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶点

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

Hello, I&acute;m not sure how to store this antibody. Datasheet says -20 &deg;C and the vial says Store at 4&deg;C. Which one is correct?

I suppose this Ab works only for rotavirus group A. Does it reacts against porcine rotavirus A as well? Are there possible crossreactions to rotavirus group B and C?

Hello, I write to ask an information about this antibody we bought recently... I have to performed an IHC-(P) on intestinal tissues...What kind of proteolitic pretratment is more suitable to unmask VP6 rotavirus protein? Trypsin? Thank you in advance!&nbsp;

Can sc-101363: Rotavirus capsid (2B4) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Hello, I´m not sure how to store this antibody. Datasheet says -20 °C and the vial says Store at 4°C. Which one is correct?

Hello, I write to ask an information about this antibody we bought recently... I have to performed an IHC-(P) on intestinal tissues...What kind of proteolitic pretratment is more suitable to unmask VP6 rotavirus protein? Trypsin? Thank you in advance!