Rock-2抗体 (30-J) 推荐用于 mouse, rat 和 human 来源的Rock-2 WB, IP, IF 和 ELISA检测

Rock-2 抗体 (30-J): sc-100425

Rock-2抗体 (30-J) is rated 4.7 out of 5 by 3.
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Datasheets

    • Rock-2抗体(30-J)是小鼠单克隆IgG2b (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组Rock-2
    • 推荐用于 mouse, rat 和 human 来源的Rock-2 WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 看 Rock-2 (D-11): sc-398519,可查看有标记的 Rock-2 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790

Which epitope is recognized by sc-100425 Rock-2 (30-J)?

Asked by: BG_Trieste
Thank you for your question. Rock-2 Antibody (30-J): sc-100425 has been raised against recombinant Rock-2 of human origin. No epitope mapping has been performed for this antibody yet, so the exact binding region of the antibody is not known.
Answered by: Tech Support Europe
Date published: 2019-06-05

I am using Rock-2 (30-J): sc-100425 for immunofluorescence, what dilution of this antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from nice quality. We have also used the antibody for IP of endogenous Rock-2 from a primary culture of mouse astrocytes,I got expected signal.It's very nice quality.
Date published: 2017-08-12
Rated 5 out of 5 by from Great WB results with HeLa whole cell lysate Great WB results with HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-27
Rated 4 out of 5 by from Produced clear immunofluorescence nuclear Produced clear immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-05
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