rnk 抗体 (464B): sc-101611

rnk Antibody (464B) is an IgG₁ mouse monoclonal rnk antibody (also designated rnk antibody) that detects the rnk protein of E. coli origin by WB and ELISA. rnk Antibody (464B) is available as the non-conjugated anti-rnk antibody. RNA polymerase transcribes DNA to synthesize RNA using the four ribonucleoside triphosphates as substrates. In prokaryotes, a catalytic core known as RNAP is formed from α, β and σ RNA pol subunits that, once complexed, can initiate transcription. The RNAP-interacting protein, rnk (regulator of nucleoside kinase) is a 136 amino acid protein that is structurally similar to the secondary channel effectors known as Gre factors. E. coli Gre factors and DksA are competitively inhibited by rnk in vitro. Cellular concentrations of rnk are similar to GreB, indicating a role as regulator of secondary channel effectors. In vitro studies indicate that rnk neither directly inhibits RNAP promoter interaction nor initiates transcript cleavage.

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References:

1. RNA polymerase structure-function: insights into points of transcriptional regulation.  |  Severinov, K. 2000. Curr Opin Microbiol. 3: 118-25. PMID: 10744988
2. Transcript cleavage factors GreA and GreB act as transient catalytic components of RNA polymerase.  |  Laptenko, O., et al. 2003. EMBO J. 22: 6322-34. PMID: 14633991
3. Effects of DksA, GreA, and GreB on transcription initiation: insights into the mechanisms of factors that bind in the secondary channel of RNA polymerase.  |  Rutherford, ST., et al. 2007. J Mol Biol. 366: 1243-57. PMID: 17207814
4. The carboxy-terminal coiled-coil of the RNA polymerase beta′-subunit is the main binding site for Gre factors.  |  Vassylyeva, MN., et al. 2007. EMBO Rep. 8: 1038-43. PMID: 17917675
5. Crystal structure of Escherichia coli Rnk, a new RNA polymerase-interacting protein.  |  Lamour, V., et al. 2008. J Mol Biol. 383: 367-79. PMID: 18760284