Rim2 抗体 (63-M7): sc-100842

Rim2 Antibody (63-M7) is an IgM mouse monoclonal Rim2 antibody that detects Rim2 of mouse, rat and human origin by WB, IP and ELISA. Rim2 Antibody (63-M7) is available as the non-conjugated anti-Rim2 antibody form. Rab3, a neural/neuroendocrine-specific member of the Rab family, is involved in Ca2+-regulated exocytosis. Rab3 functions in an inhibitory capacity by controlling the recruitment of secretory vesicles into a releasable pool at the plasma membrane. Rim (rab3 interacting molecule), a putative effector protein for Rab3s, is composed of an N-terminal zinc-finger motif and C-terminal PDZ and C2 domains. Rim exists as two variants, Rim1 and Rim2, produced by alternative splicing. The 3′-end of the Rim2 gene produces an independent mRNA that encodes a smaller protein referred to as Nim2, which like Rim, also regulates exocytosis. Rim serves as a Rab3-dependent regulator of synaptic-vesicle fusion by forming a GTP-dependent complex between synaptic plasma membranes and docked synaptic vesicles. Both Rim1 and Rim2 can bind to cAMP-GEFII, which is a direct target of cAMP in regulated exocytosis and is responsible for cAMP-dependent, PKA-dependent exocytosis. Rim also localizes on the plasma membrane of INS-1E cells and pancreatic beta-cells. Rab3 binding domain of Rim enhances glucose-stimulated secretion in intact cells and Ca2+-stimulated exocytosis in permeabilized cells, suggesting that Rim may also play a regulatory role in insulin secretion.

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References:

1. Disruption of Rab3-calmodulin interaction, but not other effector interactions, prevents Rab3 inhibition of exocytosis.  |  Coppola, T., et al. 1999. EMBO J. 18: 5885-91. PMID: 10545100
2. The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins.  |  Wang, Y., et al. 2000. J Biol Chem. 275: 20033-44. PMID: 10748113
3. The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis.  |  Iezzi, M., et al. 2000. FEBS Lett. 474: 66-70. PMID: 10828453
4. cAMP-GEFII is a direct target of cAMP in regulated exocytosis.  |  Ozaki, N., et al. 2000. Nat Cell Biol. 2: 805-11. PMID: 11056535
5. A direct inhibitory role for the Rab3-specific effector, Noc2, in Ca2+-regulated exocytosis in neuroendocrine cells.  |  Haynes, LP., et al. 2001. J Biol Chem. 276: 9726-32. PMID: 11134008
6. Rim is a putative Rab3 effector in regulating synaptic-vesicle fusion.  |  Wang, Y., et al. 1997. Nature. 388: 593-8. PMID: 9252191