Ribosomal Protein S14抗体 (3G5) 推荐用于 mouse, rat 和 human 来源的Ribosomal Protein S14 WB, IP 和 ELISA检测

Ribosomal Protein S14 抗体 (3G5): sc-293478

Ribosomal Protein S14抗体 (3G5) is rated 5.0 out of 5 by 3.
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Datasheets

    • 抗-Ribosomal Protein S14 抗体 3G5 是小鼠单克隆 IgG2a κ,Ribosomal Protein S14抗体, 在2篇文献中引用,规格为100 µg/ml
    • raised against amino acids 45-144 representing partial length Ribosomal Protein S14 of human origin
    • 推荐用于 mouse, rat 和 human 来源的Ribosomal Protein S14 WB, IP 和 ELISA检测
    • 关于如何获取Ribosomal Protein S14 (3G5): sc-293478免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 目前,我们还没有完成Ribosomal Protein S14 抗体 (3G5)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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你们的抗体序列是全长还是部分

Asked by: hailiang
感谢您的提问。sc-293478 Ribosomal Protein S14 (3G5)是针对人源核糖体蛋白S14的部分氨基酸长度作为抗原生产的抗体,抗原氨基酸序列为45-144 Thank you for your question. sc-293478 Ribosomal Protein S14 (3G5) is raised against amino acids 45-144 representing partial length Ribosomal Protein S14 of human origin.
Answered by: Technical Support 8
Date published: 2018-12-21

i work on zebrafish and generally only use polyclonal antibodies for this reason. there is only a single amino acid difference between the human and zebrafish in this region . do you know how likely this is to work? Happy to test an aliquot 

Asked by: Bethpay
If you are working with a cleaved or modified protein or with a protein from a different animal species and if you are concerned about reactivity, please send us your sequence or the protein accession number ([email protected]). We will be more than happy to check the homology and let you know if any of our antibodies will be appropriate for your experiments.
Answered by: Technical Support Europe
Date published: 2017-06-28

What is the recommended starting dilution for Western blot for Ribosomal Protein S14 (3G5): sc-293478 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-293478.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-04-05
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Rated 5 out of 5 by from Nice specificity! Detects human and Neurospora crassa RPS14 readily (does not recognize yeast RPS14). Protein easily detected in Western blot (50 µg of total protein, primary Ab diluted 1:200, secondary antibody diluted 1:10,000, detection with Super Signal Pico).
Date published: 2018-02-20
Rated 5 out of 5 by from a fantastic antibody for WB This antibody recognizes a very clear and strong band of predicted M.W. on the western blot (1:500 dilution, 40 ug U251 lysate).
Date published: 2017-08-28
Rated 5 out of 5 by from Good specificity in WB in target band! In target region of the membrane, this antibody shows a good specificity in WB in PLC whole cell lysate.
Date published: 2017-03-31
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