Ribosomal Protein L19抗体 (K-12) 推荐用于 mouse, rat 和 human 来源的Ribosomal Protein L19 WB, IP, IF, IHC(P) 和 ELISA检测

Ribosomal Protein L19 抗体 (K-12): sc-100830

Ribosomal Protein L19抗体 (K-12) is rated 4.6 out of 5 by 7.
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Datasheets

    • 抗-Ribosomal Protein L19抗体(K-12)是小鼠单克隆IgG2a (lambda轻链) Ribosomal Protein L19抗体,规格为100 µg/ml
    • raised against recombinant protein mapping within amino acids 1-101 of Ribosomal Protein L19 of human origin
    • 推荐用于 mouse, rat 和 human 来源的Ribosomal Protein L19 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP)
    • 关于如何获取Ribosomal Protein L19 (K-12): sc-100830免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Ribosomal Protein L19 (K-12): sc-100830 monoclonal antibody?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGλ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works but relatively weak Purchased for IF application. HEK cell fixed in Methanol (top) and 4% Formaldehyde (bottom) stained with 1:100 @RT for 1hr. Used sc-516190 for 2Ab. It worked in both stain which makes me happy, but signal was pretty weak. (I am using super high gage here...) Also, be aware that this is LAMBDA FRAGMENT. If you are using recent SCBT secondary antibody, most likely it is Kappa which will not bind to lambda. Use either sc-516190, or general anti-mouse (H+L) so that it has affinity to lambda fragment.
Date published: 2019-04-10
Rated 5 out of 5 by from Good signal on WB I tested this antibody in mouse pancreatic cancer line. This produced strong and specific signals (5% milk, 1:200).
Date published: 2018-11-13
Rated 3 out of 5 by from not optimal Tested this antibody at the same time as several other monoclonal antibodies against ribosomal proteins. This one had the weakest signal, and the most non-specific bands showing up in Western blots.
Date published: 2018-02-21
Rated 5 out of 5 by from Good publishable data in WB in mouse testes Good publishable data in WB in mouse testes. -SCBT Publication Review
Date published: 2015-05-13
Rated 5 out of 5 by from Strong signal in WB in mouse transfected Strong signal in WB in mouse transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Single band in WB in HeLa whole cell lysate Single band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human small Intestine tissue. -SCBT QC
Date published: 2015-01-24
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