Ret抗体 (6E4C4) 推荐用于 mouse, rat 和 human 来源的Ret WB, IP, IF, IHC(P) 和 ELISA检测

Ret 抗体 (6E4C4): sc-101423

Ret抗体 (6E4C4) is rated 3.7 out of 5 by 3.
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Datasheets

    • Ret抗体(6E4C4)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 896-1063 of Ret of human origin
    • 抗-Ret 抗体 (6E4C4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Ret
    • 抗-Ret 抗体 (6E4C4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Ret (6E4C4): sc-101423免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

I am using Ret (6E4C4): sc-101423 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good results This antibody was used as a capture antibody for endogenous mouse RET in a sandwich ELISA. We got good results.
Date published: 2017-02-08
Rated 3 out of 5 by from Strong band seen in WB using human truncated Strong band seen in WB using human truncated recombinant Ret fusion protein. -SCBT QC
Date published: 2015-01-25
Rated 3 out of 5 by from Publishable data in IHC(P) using human aborted Publishable data in IHC(P) using human aborted fetuses, ovarian biopsy -SCBT Publication Review
Date published: 2013-06-19
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