Ret 抗体 (6E4C4): sc-101423

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Datasheets

- Ret抗体(6E4C4)是小鼠单克隆IgG_2b κ, 在2篇文献中引用，规格为200 µg/ml
- raised against a recombinant protein corresponding to amino acids 896-1063 of Ret of human origin
- 抗-Ret 抗体 (6E4C4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Ret
- 抗-Ret 抗体 (6E4C4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取Ret (6E4C4): sc-101423的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG_2b BP-HRP 和 m-IgGκ BP-HRP 是 Ret Antibody (6E4C4) for WB and IHC(P) 首选应用二抗. 这些试剂现在以套形式提供 Ret Antibody (6E4C4) (见下方订购信息).

SEE ALSO...

ChemCruz™ Chemical Ret Inhibitors可用于Ret细胞反应的功能性分析

Ret Antibody (6E4C4) - Immunohistochemistry - Image 356403

Ret Antibody (6E4C4) - Western Blotting - Image 361003

Ret (6E4C4) Alexa Fluor® 488: sc-101423 AF488. Direct immunofluorescence staining of...

Ret Antibody (6E4C4) - Immunohistochemistry - Image 356402

Ret Antibody (6E4C4) - Western Blotting - Image 400095

Ret Antibody (6E4C4) - Western Blotting - Image 400094

Ret Antibody (6E4C4) - Western Blotting - Image 400093

Ret Antibody (6E4C4) - Western Blotting - Image 400096

Ret Antibody (6E4C4) - Western Blotting - Image 361002

Ret Antibody (6E4C4) - Western Blotting - Image 392737

Ret Antibody (6E4C4) - Western Blotting - Image 127433

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Ret Antibody (6E4C4) is an IgG2b κ mouse monoclonal Ret antibody suitable for the detection of Ret of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Ret Antibody (6E4C4) is available as the non-conjugated anti-Ret antibody form, as well as multiple conjugated forms of anti-Ret antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The Ret proto-oncogene is structurally related to the growing family of tyrosine kinase transmembrane receptors and is involved in GDNF signaling. By alternative splicing, two isoforms of the Ret proto-oncogene product are generated. The isoforms differ from each other by having either 9 or 51 carboxy terminal amino acids. The Ret gene products include two glycosylated proteins and, in tunicamycin treated cells, a non-glycosylated protein consistent with the predicted Ret molecular weight based on sequence analysis. Tumor-specific rearrangements of the Ret proto-oncogene have been identified in papillary thyroid carcinomas leading to the formation of different transforming fusion proteins sharing the tyrosine kinase domain of Ret. In contrast to the Ret proto-oncogene, the rearranged forms are constitutively phosphorylated on tyrosine and are translocated from the membrane to the cytoplasm.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	RET	Entrez Gene ID: 5979	10q11.21	NM_020630, NM_020975, NM_000323, NM_020629	UniProt ID: P07949	209880
Mouse	Ret	Entrez Gene ID: 19713	6 F1	NM_001080780, NM_009050	UniProt ID: P35546	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗Ret抗体(6E4C4)在各种科学出版物中有2篇引文。

针对哺乳动物靶标蛋白的一抗，如抗Ret 抗体 (6E4C4)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

Ret 抗体 (6E4C4) Specifications

替代产品名称

Ret

产品克隆名称

6E4C4

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2b kappa light chain

氨基酸

896-1063

抗原表位靶标

Ret

抗原表位种属

human

产品反应性/检测

Ret

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

2篇各种科学出版物的引文

可选的偶联物/形式

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

果蝇

非洲爪蟾蜍

拟南芥

秀丽隐杆线虫

梭菌

弯曲杆菌

幽门螺杆菌

肝炎

克雷白氏杆菌

结核分支杆菌

疟原虫

假单胞菌

酿酒酵母

沙门氏菌

血吸虫

志贺氏杆菌

葡萄球菌

優選的 Ret 抗體是 Ret 抗體 (C-3): sc-365943.

I am using Ret (6E4C4): sc-101423 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp

We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by snowy from good results This antibody was used as a capture antibody for endogenous mouse RET in a sandwich ELISA. We got good results.

Date published: 2017-02-08

Rated 3 out of 5 by ET from Strong band seen in WB using human truncated Strong band seen in WB using human truncated recombinant Ret fusion protein. -SCBT QC

Date published: 2015-01-25

Rated 3 out of 5 by FL from Publishable data in IHC(P) using human aborted Publishable data in IHC(P) using human aborted fetuses, ovarian biopsy -SCBT Publication Review

Date published: 2013-06-19

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