RBMS1 抗体 (73-K2): sc-101190

RBMS1 Antibody (73-K2) is an IgG₁ κ mouse monoclonal RBMS1 antibody (also designated MSSP antibody or SCR2 antibody) that detects the RBMS1 protein of mouse, rat and human origin by WB, IP and ELISA. RBMS1 Antibody (73-K2) is available as the non-conjugated anti-RBMS1 antibody. RBMS1 (RNA binding motif, single stranded interacting protein 1), also known as YC1, MSSP (c-Myc single strand binding protein), SCR2 (suppressor of Cdc2 with RNA binding motif), MSSP-1, MSSP-2 or MSSP-3, is a member of the MSSP family of proteins. The MSSP family is comprised of proteins that bind to single stranded DNA/RNA. Through an interaction with the c-Myc protein, members of this family are involved in a wide variety of cellular functions, including gene transcription, DNA replication, apoptosis and cell cycle progression. RBMS1, a nuclear localized protein, is expressed in lung, placenta and heart with highest expression levels during the G1 to S transition phase of the cell cycle. RBMS1 contains two RNP domains, namely RNP1-A and RNP1-B, both of which are necessary for DNA binding. RBMS1 specifically binds to a catalytic subunit of DNA polymerase (pol) α and stimulates its activity in vitro. Due to alternative splicing events, various isoforms exist for RBMS1.

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References:

1. MSSP, a protein binding to an origin of replication in the c-myc gene, interacts with a catalytic subunit of DNA polymerase alpha and stimulates its polymerase activity.  |  Niki, T., et al. 2000. FEBS Lett. 475: 209-12. PMID: 10869558
2. RNA-binding protein RBMS3 is expressed in activated hepatic stellate cells and liver fibrosis and increases expression of transcription factor Prx1.  |  Fritz, D. and Stefanovic, B. 2007. J Mol Biol. 371: 585-95. PMID: 17586524
3. Molecular cloning of MSSP-2, a c-myc gene single-strand binding protein: characterization of binding specificity and DNA replication activity.  |  Takai, T., et al. 1994. Nucleic Acids Res. 22: 5576-81. PMID: 7838710
4. SCR: novel human suppressors of cdc2/cdc13 mutants of Schizosaccharomyces pombe harbour motifs for RNA binding proteins.  |  Kanaoka, Y. and Nojima, H. 1994. Nucleic Acids Res. 22: 2687-93. PMID: 8041632
5. Identification and cDNA cloning of single-stranded DNA binding proteins that interact with the region upstream of the human c-myc gene.  |  Negishi, Y., et al. 1994. Oncogene. 9: 1133-43. PMID: 8134115
6. Cloning and characterization of the genomic DNA of the human MSSP genes.  |  Haigermoser, C., et al. 1996. Nucleic Acids Res. 24: 3846-57. PMID: 8871567