Rb抗体 (IF8) 推荐用于 mouse, rat 和 human 来源的Rb p110 WB, IP, IF, IHC(P) 和 FCM检测

Rb 抗体 (IF8): sc-102

Rb抗体 (IF8) is rated 4.7 out of 5 by 27.
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Datasheets

    • 抗-Rb 抗体 IF8 是小鼠单克隆 IgG1 (kappa轻链),Rb抗体,规格为200 µg/ml
    • 免疫成视网膜细胞瘤基因产物/β-galactosidase融合蛋白
    • 抗-Rb 抗体 (IF8) 推荐用于 WB, IP, IF, IHC(P) 和 FCM,检测mouse, rat 和human 来源的 Rb p110
    • 抗-Rb 抗体 (IF8) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Rb (IF8): sc-102免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Do you happen to know whether this mAb (IF8) is reactive with pig (porcine) RB by IHC and WB, looking for a pig RB antibody? Thanks!

Asked by: JWS1
Thank you for your question. Rb Antibody (IF8): sc-102 is recommended for detection of Rb p110 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. We have no internal data for this antibody's reactivity in porcine tissues and it is not recommended for this use. However there is a publication in which this antibody was used for detection of Rb in pig. The corresponding PMID is 16293970.
Answered by: Tech Support Europe
Date published: 2019-07-11

Hello My name is Laura, I bought the antibody sc-102 Rb and I am getting two bands, I need to know... ¿Is it a phosphorylated form of Rb or a nonspecific band? thank you so much

Asked by: Lauralopezbanuelos
Thank you for your question. We would recommend treating your lysates with Lambda Phosphatase: sc-200312A to confirm whether or not the antibody is specifically detecting phosphorylated Rb in your samples.
Answered by: Technical Support
Date published: 2019-01-26

I'm interested in sc-102 and would like to receive a FREE 10 µg sample of Rb (IF8): sc-102 for testing. Is this possible ?

Asked by: Janine Kruit
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Is the epitope that is recognised by the antibody known? (which aa region?) 

Asked by: ResearcherfromLeuven
Thank you for your inquiry. The epitope region is not known. For additional questions, please contact our Technical Service by email ([email protected]) or phone (00800 4573 8000).
Answered by: Technical Support Europe
Date published: 2017-12-20

Does this antibody (SC_102) bind with other proteins in Rb family? I can detect three bands of which their M.W. are within range of protein marker from 100 to 150 kD. Are all these bands Rb protein? (protein lysate from MCF7, 293T; Ab dilution 1:800)

Asked by: Wareerat
Thank you for your question. This mouse monoclonal antibody, Rb (IF8): sc-102, is specific for the detection of Rb. It sounds like your results are inconsistent with our Western blot results published on our website for this antibody. We would recommend you include a secondary antibody control (omit primary antibody incubation) to confirm if any of the other bands you are seeing are due to non-specific binding of the secondary antibody alone. Please contact our Technical Service Department for further help in troubleshooting your Western blot results with this antibody. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-11-15

Does this "Rb Antibody (IF8)" bind with both the phosphorylated and the un-phosphorylated Rb proteins? I can then detect the relative number of the phosphorylated Rb with one of the p-Rb antibodies, is this correct? Thanks.

Asked by: blcxfk
Thank you for your question. This mouse monoclonal antibody, Rb (IF8): sc-102, is not phospho-specific and therefore should be able to detect both the un-phosphorylated and phosphorylated Rb protein. If you use this antibody as a control along with a phospho-specific Rb antibody you will observe a qualitative but not a quantitative difference by Western blot.
Answered by: Technical Support
Date published: 2019-11-30

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 4 out of 5 by from works well for WB I used this antibody for WB and obtained good results.
Date published: 2020-08-21
Rated 5 out of 5 by from To recommend Monoclonal IF8 was obtained from S. Cruz as complimentary antibody. It was used at dilution 1/200 to probe whole cell extracts (50µg) from nocodazole-synchronized U2OS resolved on 7.5% SDS-PAGE Anderson gel system. M: mitosis; G1: G1 phase.
Date published: 2019-04-10
Rated 5 out of 5 by from good product This antibody works well for western blot experiment.
Date published: 2018-08-22
Rated 5 out of 5 by from Great antibody! Specific and low background. I see two bands close to each other. they might be phosphorylated and un-phosphoyrlated forms.
Date published: 2018-03-01
Rated 5 out of 5 by from Works well for WB works very well for western - samples were human cells in culture
Date published: 2017-11-21
Rated 3 out of 5 by from Good antibody for western blot to dilute 250x? The batch of antibody I have worked well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific but very weak. The antibody was diluted 500 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2017-11-16
Rated 5 out of 5 by from Great Works well at 1:250 in LNCaP cell lysate. Detects approximately 110 kDa in 8% PAGE
Date published: 2017-09-21
Rated 5 out of 5 by from Great for western blots Gives clean band at the expected size diluted 1:500 in milk
Date published: 2017-09-20
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