QIP1 抗体 (3D10): sc-101547

QIP1 Antibody (3D10) is an IgG_2a rat monoclonal QIP1 antibody (also designated QIP1 antibody) suitable for the detection of the QIP1 protein of mouse, rat, human, hamster, bovine and monkey origin by WB, IP, IF, IHC(P) and ELISA. QIP1 Antibody (3D10) is available as both the non-conjugated anti-QIP1 antibody form, as well as multiple conjugated forms of anti-QIP1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. QIP1, also known as KPNA4 (karyopherin α4), IPOA3 (importin α3) or SRP3, is a member of the importin α family. It is involved in nuclear import and forms a complex with the importin β protein, karyopherin β1, functioning as its adapter protein. QIP1 binds to substrates containing nuclear localization signal (NLS) motifs, while karyopherin β1 facilitates the binding of the importin/substrate complex to the nuclear pore complex (NPC). Subsequently, the importin/substrate complex is translocated through the pore via a Ran-dependent mechanism. QIP1 contains one IBB domain at its hydrophilic N-terminus which is required for binding karyopherin β1 and ten ARM repeats in its hydrophobic central region. QIP1 is expressed at high levels in pancreas, lung, ovary, testis, small intestine, heart and skeletal muscle, exhibiting both cytoplasmic and nuclear localization.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Evidence for distinct substrate specificities of importin alpha family members in nuclear protein import.  |  Köhler, M., et al. 1999. Mol Cell Biol. 19: 7782-91. PMID: 10523667
2. Assignment of KPNA4 and KPNB1 encoding karyopherin alpha 4 and beta 1 to human chromosome bands 11q22 and 17q21 respectively, by in situ hybridization.  |  Ayala-Madrigal, ML., et al. 2000. Cytogenet Cell Genet. 89: 258-9. PMID: 10965137
3. Generation of a rat monoclonal antibody specific for importin alpha3/Qip1.  |  Sakaguchi, N., et al. 2003. Hybrid Hybridomics. 22: 397-400. PMID: 14683601
4. The nuclear import of RNA helicase A is mediated by importin-alpha3.  |  Aratani, S., et al. 2006. Biochem Biophys Res Commun. 340: 125-33. PMID: 16375861
5. Differential regulation of karyopherin alpha 2 expression by TGF-beta1 and IFN-gamma in normal human epidermal keratinocytes: evident contribution of KPNA2 for nuclear translocation of IRF-1.  |  Umegaki, N., et al. 2007. J Invest Dermatol. 127: 1456-64. PMID: 17255955
6. Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL.  |  Seki, T., et al. 1997. Biochem Biophys Res Commun. 234: 48-53. PMID: 9168958
7. Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors.  |  Miyamoto, Y., et al. 1997. J Biol Chem. 272: 26375-81. PMID: 9334211
8. Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family.  |  Köhler, M., et al. 1997. FEBS Lett. 417: 104-8. PMID: 9395085