PSIP1 抗体 (3F7): sc-101087

PSIP1 Antibody (3F7) is an IgG₁ κ mouse monoclonal PSIP1 antibody (also designated PSIP1 antibody) that detects the PSIP1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PSIP1 Antibody (3F7) is available as the non-conjugated anti-PSIP1 antibody. PSIP1 (PC4 and SFRS1 interacting protein 1), also known as CLL-associated antigen KW-7, PSIP2, LEDGF (lens epithelium-derived growth factor), PAIP, DFS70 (dense fine speckles 70 kDa protein) or transcriptional coactivator p75/p52, is a 530 amino acid nuclear protein that associates with chromatin throughout the cell cycle. Functioning as a transcriptional coactivator that complexes with the human immunodeficiency virus type 1 (HIV-1) integrase, PSIP1 is essential for the nuclear localization and chromosomal association of viral proteins. As the primary integrase-to-chromatin tethering factor for HIV-1, PSIP1 is responsible for the cellular trafficking of lentiviral integrases. During apoptosis, PSIP1 is cleaved at three sites by caspase-3 and caspase-7, contributing to the pathogenesis of atopic disorders.

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References:

1. Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing.  |  Singh, DP., et al. 2000. Gene. 242: 265-73. PMID: 10721720
2. Caspase cleavage of the nuclear autoantigen LEDGF/p75 abrogates its pro-survival function: implications for autoimmunity in atopic disorders.  |  Wu, X., et al. 2002. Cell Death Differ. 9: 915-25. PMID: 12181742
3. Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX.  |  Krackhardt, AM., et al. 2002. Blood. 100: 2123-31. PMID: 12200376
4. HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells.  |  Cherepanov, P., et al. 2003. J Biol Chem. 278: 372-81. PMID: 12407101
5. LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes.  |  Llano, M., et al. 2004. J Virol. 78: 9524-37. PMID: 15308744
6. Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase.  |  Cherepanov, P., et al. 2004. J Biol Chem. 279: 48883-92. PMID: 15371438
7. Lens epithelium-derived growth factor/p75 prevents proteasomal degradation of HIV-1 integrase.  |  Llano, M., et al. 2004. J Biol Chem. 279: 55570-7. PMID: 15475359
8. Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering.  |  Vanegas, M., et al. 2005. J Cell Sci. 118: 1733-43. PMID: 15797927
9. Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation.  |  Ge, H., et al. 1998. EMBO J. 17: 6723-9. PMID: 9822615