PSF 抗体 (39-1): sc-101137

PSF Antibody (39-1) is an IgG_2a κ mouse monoclonal PSF antibody (also designated Splicing Factor Proline And Glutamine Rich antibody, PSF antibody, Polypyrimidine Tract Binding Protein Associated antibody, Protein Phosphatase 1 Regulatory Subunit 140 antibody, DNA-Binding P52/P100 Complex 100 KDa Subunit antibody, Splicing Factor Proline- And Glutamine-Rich antibody, PPP1R140 antibody, Polypyrimidine Tract-Binding Protein-Associated Splicing Factor antibody, or PTB-Associated-Splicing Factor antibody) that detects the PSF protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PSF Antibody (39-1) is available as the non-conjugated anti-PSF antibody. Pre-mRNA splicing is a critical step in the post-translational regulation of gene expression. The process of removing intron sequences from mRNA is a two-step enzymatic reaction that requires the action of the spliceosome, a large multicomponent ribonucleoprotein complex. The polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF) is a ubiquitously expressed protein that forms a complex with PTB, also designated hnRNP I, which is required for early spliceosome formation and is essential for catalytic step II. The PSF protein contains two RNA recognition motifs (RRMs), a proline- and glutamine-rich amino terminal domain, and two carboxy-terminal nuclear localization signals. PSF is localized to the nucleus in punctate structures called speckles, which are absent from nucleoli. The localization of PSF to speckles is dependent upon the presence of the second RRM motif. PSF also can associate with the DNA binding domains (DBDs) of thyroid hormone receptors and retinoic acid receptors, where it acts as a repressor by recruiting HDACs to the DBDs. PSF is expressed in neurons during development and may be involved in neuronal differentiation and maturation. PSF is proteolytically cleaved to produce a shorter fragment in myeloid cells.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Identification of PSF, the polypyrimidine tract-binding protein-associated splicing factor, as a developmentally regulated neuronal protein.  |  Chanas-Sacrε, G., et al. 1999. J Neurosci Res. 57: 62-73. PMID: 10397636
2. Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB.  |  Meissner, M., et al. 2000. J Cell Biochem. 76: 559-66. PMID: 10653975
3. Enhanced proteolysis of pre-mRNA splicing factors in myeloid cells.  |  Shav-Tal, Y., et al. 2000. Exp Hematol. 28: 1029-38. PMID: 11008015
4. An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles.  |  Dye, BT. and Patton, JG. 2001. Exp Cell Res. 263: 131-44. PMID: 11161712
5. PSF is a novel corepressor that mediates its effect through Sin3A and the DNA binding domain of nuclear hormone receptors.  |  Mathur, M., et al. 2001. Mol Cell Biol. 21: 2298-311. PMID: 11259580
6. A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction.  |  Gozani, O., et al. 1994. EMBO J. 13: 3356-67. PMID: 8045264
7. Cloning and characterization of PSF, a novel pre-mRNA splicing factor.  |  Patton, JG., et al. 1993. Genes Dev. 7: 393-406. PMID: 8449401