POH1 抗体 (SQ-17): sc-100464

POH1 Antibody (SQ-17) is an IgG₁ κ mouse monoclonal POH1 antibody (also designated POH1 antibody) suitable for the detection of the POH1 protein of mouse, rat and human origin by WB, IP, IF and ELISA. POH1 Antibody (SQ-17) is available as the non-conjugated anti-POH1 antibody. POH1, the human homolog of yeast Pad1, is part of the 26 S proteasome which degrades protein targeted for destruction by the ubiquitin pathway. Specifically, POH1 is part of the 19S regulatory cap of the 26S proteasome where it deubiquitinates proteins and allows proteins to pass through the narrow, cylindrical 26 S proteasome core. POH1 is most abundantly expressed in heart and skeletal muscle. Transient overexpression of POH1 in mammalian cells confers P-glycoprotein-indedependent resistance to taxol, doxorubicin, 7-hydroxystaurosporine and ultraviolet light. The gene encoding human POH1 maps to chromosome 2q24.2. The Pad1 homolog in marine sponges (POHL) is upregulated in response to toxins staurosporin and taxol. In Schistosoma mansoni, the Pad1 homolog SmPOH appears to stabilize c-Jun and elevates the levels of c-Jun for transactivation of AP-1-responsive genes.

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References:

1. Potential multidrug resistance gene POHL: an ecologically relevant indicator in marine sponges.  |  Krasko, A., et al. 2001. Environ Toxicol Chem. 20: 198-204. PMID: 11351409
2. A Schistosoma mansoni Pad1 homologue stabilizes c-Jun.  |  Nabhan, JF., et al. 2001. Mol Biochem Parasitol. 116: 209-18. PMID: 11522353
3. A cryptic protease couples deubiquitination and degradation by the proteasome.  |  Yao, T. and Cohen, RE. 2002. Nature. 419: 403-7. PMID: 12353037
4. Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit.  |  Spataro, V., et al. 1997. J Biol Chem. 272: 30470-5. PMID: 9374539