Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
POH1 Antibody (SQ-17) is a high quality monoclonal POH1 antibody (also designated POH1 antibody) suitable for the detection of the POH1 protein of mouse, rat and human origin. POH1 Antibody (SQ-17) is available as the non-conjugated anti-POH1 antibody. POH1, the human homolog of yeast Pad1, is part of the 26 S proteasome which degrades protein targeted for destruction by the ubiquitin pathway. Specifically, POH1 is part of the 19S regulatory cap of the 26S proteasome where it deubiquitinates proteins and allows proteins to pass through the narrow, cylindrical 26 S proteasome core. POH1 is most abundantly expressed in heart and skeletal muscle. Transient overexpression of POH1 in mammalian cells confers P-glycoprotein-indedependent resistance to taxol, doxorubicin, 7-hydroxystaurosporine and ultraviolet light. The gene encoding human POH1 maps to chromosome 2q24.2. The Pad1 homolog in marine sponges (POHL) is upregulated in response to toxins staurosporin and taxol. In Schistosoma mansoni, the Pad1 homolog SmPOH appears to stabilize c-Jun and elevates the levels of c-Jun for transactivation of AP-1-responsive genes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using POH1 (SQ-17): sc-100464 for immunofluorescence, what dilution of this antibody should I use?
Asked by: jerojero
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: