PIP5KIII 抗体 (64-Q6): sc-100408

PIP5KIII Antibody (64-Q6) is an IgG_2a κ mouse monoclonal PIP5KIII antibody (also designated PIP5KIII antibody) suitable for the detection of the PIP5KIII protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PIP5KIII Antibody (64-Q6) is available as the non-conjugated anti-PIP5KIII antibody. Phosphorylation of phosphatidylinositol (PtdIns) derivatives is suggested to play a role in regulating cytoskeletal functions, membrane trafficking and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. PtdIns proteins are distinguished by the degree and position of phosphorylation of the inositol ring. PIP5KIII (Phosphatidylinositol 3-phosphate 5-kinase), also known as PIP5K3, FAB1, CFD, PIKFYVE or ZFYVE29 is 2,098 amino acid protein that synthesizes phosphatidylinositol-3,5-bisphosphate by catalyzing the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the inositol ring. PIP5KIII is thought to play a major role in nuclear migration and the endocytic-vacuolar pathway. PIP5KIII exists as four alternatively spliced isoforms and contains a DEP domain, a FYVE-type zinc finger and a PIPK domain. Defects in the gene encoding PIP5KIII lead to corneal fleck dystrophy (CFD), an autosomal disorder in which small white flecks are found throughout the corneal stroma..

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References:

1. Phosphatidylinositol 3-phosphate-interacting domains in PIKfyve. Binding specificity and role in PIKfyve. Endomenbrane localization.  |  Sbrissa, D., et al. 2002. J Biol Chem. 277: 6073-9. PMID: 11706043
2. PIKfyve controls fluid phase endocytosis but not recycling/degradation of endocytosed receptors or sorting of procathepsin D by regulating multivesicular body morphogenesis.  |  Ikonomov, OC., et al. 2003. Mol Biol Cell. 14: 4581-91. PMID: 14551253
3. The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport.  |  Rutherford, AC., et al. 2006. J Cell Sci. 119: 3944-57. PMID: 16954148
4. The phosphoinositide kinase PIKfyve mediates epidermal growth factor receptor trafficking to the nucleus.  |  Kim, J., et al. 2007. Cancer Res. 67: 9229-37. PMID: 17909029
5. PIKfyve: Partners, significance, debates and paradoxes.  |  Shisheva, A. 2008. Cell Biol Int. 32: 591-604. PMID: 18304842
6. Elevated levels of PtdIns5P in NPM-ALK transformed cells: implication of PIKfyve.  |  Coronas, S., et al. 2008. Biochem Biophys Res Commun. 372: 351-5. PMID: 18501703
7. PIKfyve regulation of endosome-linked pathways.  |  de Lartigue, J., et al. 2009. Traffic. 10: 883-93. PMID: 19582903
8. A novel PIKFYVE mutation in fleck corneal dystrophy.  |  Kotoulas, A., et al. 2011. Mol Vis. 17: 2776-81. PMID: 22065932
9. Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration.  |  Oppelt, A., et al. 2013. EMBO Rep. 14: 57-64. PMID: 23154468