PIP5KIII 抗体 (64-Q6): sc-100408

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- PIP5KIII 抗体 64-Q6 是小鼠单克隆 IgG_2a κ，PIP5KIII抗体, 在10篇文献中引用，规格为100 µg/ml
- raised against amino acids 342-452 mapping within an internal region of PIP5KIII of human origin
- 推荐用于 mouse, rat 和 human 来源的PIP5KIII WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取PIP5KIII (64-Q6): sc-100408的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP and m-IgG_2a BP-HRP 是 PIP5KIII Antibody (64-Q6) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供PIP5KIII Antibody (64-Q6) (见下方订购信息).

SEE ALSO...

ChemCruz™ Chemical PIP5KIII Inhibitors可用于PIP5KIII细胞反应的功能性分析

PIP5KIII Antibody (64-Q6) - Western Blotting - Image 368258

PIP5KIII Antibody (64-Q6) - Immunohistochemistry - Image 36010

PIP5KIII Antibody (64-Q6) - Immunofluorescence - Image 35557

PIP5KIII Antibody (64-Q6) - Western Blotting - Image 50435

PIP5KIII Antibody (64-Q6) - Western Blotting - Image 31680

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PIP5KIII Antibody (64-Q6) is a high quality monoclonal PIP5KIII antibody (also designated PIP5KIII antibody) suitable for the detection of the PIP5KIII protein of mouse, rat and human origin. PIP5KIII Antibody (64-Q6) is available as the non-conjugated anti-PIP5KIII antibody. Phosphorylation of phosphatidylinositol (PtdIns) derivatives is suggested to play a role in regulating cytoskeletal functions, membrane trafficking and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. PtdIns proteins are distinguished by the degree and position of phosphorylation of the inositol ring. PIP5KIII (Phosphatidylinositol 3-phosphate 5-kinase), also known as PIP5K3, FAB1, CFD, PIKFYVE or ZFYVE29 is 2,098 amino acid protein that synthesizes phosphatidylinositol-3,5-bisphosphate by catalyzing the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the inositol ring. PIP5KIII is thought to play a major role in nuclear migration and the endocytic-vacuolar pathway. PIP5KIII exists as four alternatively spliced isoforms and contains a DEP domain, a FYVE-type zinc finger and a PIPK domain. Defects in the gene encoding PIP5KIII lead to corneal fleck dystrophy (CFD), an autosomal disorder in which small white flecks are found throughout the corneal stroma..

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	PIKFYVE	200576	2q34	XM_011510778, XM_011510779, XM_011510780, XM_011510781, XM_011510782, XM_011510783, XM_011510784, XM_011510785, XM_011510786, XM_011510787, XM_011510788, XM_011510789, XM_011510790, XM_011510792, XM_017003568, XM_017003569, XM_017003570, XM_017003571, XM_017003572, XM_017003573, XM_017003574, NM_001178000, NM_015040, NM_152671, NM_001002881	Q9Y2I7	609414
Mouse	Pikfyve	18711	1 C2	XM_006495776, XM_006495777, XM_006495779, XM_006495781, XM_006495782, XM_011238454, NM_001310624, NM_011086	Q9Z1T6	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗PIP5KIII抗体(64-Q6)在各种科学出版物中有10篇引文。

针对哺乳动物靶标蛋白的一抗，如抗PIP5KIII 抗体 (64-Q6)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

PIP5KIII 抗体 (64-Q6) Specifications

替代产品名称

PIP5KIII

产品克隆名称

64-Q6

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2a kappa light chain

氨基酸

342-452

抗原表位靶标

PIP5KIII

抗原表位种属

human

产品反应性/检测

PIP5KIII

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

10篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗PIP5KIII抗体(64-Q6)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with PIP5KIII (64-Q6): sc-100408 monoclonal antibody?

Asked by: TinTin

We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety

Answered by: Technical Support 5

Date published: 2017-01-25

Rated 4 out of 5 by user_01 from Good antibody worked well at 1:250 dilution in human primary macrophages

Date published: 2017-11-01

Rated 5 out of 5 by Professor Griffin from Great for staining Nice strong and specific staining observed with PIP5KIII Antibody (64-Q6)

Date published: 2017-01-17

Rated 5 out of 5 by TS from IP and WB publishable data with mouse NIH IP and WB publishable data with mouse NIH-3T3 - SCBT Publication Review

Date published: 2015-04-06

Rated 5 out of 5 by ET from Beautiful immunoperoxidase membrane and cytoplasmic Beautiful immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human testis tissue. -SCBT QC

Date published: 2014-11-20

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PIP5KIII 抗体 (64-Q6): sc-100408

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PIP5KIII 抗体 (64-Q6) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with PIP5KIII (64-Q6): sc-100408 monoclonal antibody?