使用 PhosphoCruzTM 蛋白纯化系统 sc-24964 处理经 EGF 处理的 A-431 细胞. 图 1: 用磷酸化酪氨酸抗体 sc-508 对印迹进行检测, 以检测酪氨酸磷酸化的表皮生长因子受体. A/B泳道: 未结合的色谱柱馏分. C/D: 结合柱 (富含磷蛋白) 馏分. E/F Lanes E/F: 分别从未经处理和经 EGF 处理的培养物中制备的全细胞裂解液. 图 2: 用 HSP 60 (sc-13115) 抗体分析图 1 所示的相同样本.
PhosphoCruz™ Protein Purification System: sc-24964
- affinity purification system devised to enrich/isolate phosphoproteins from cell lysates and tissue extracts for phosphorylation studies; proteins that carry a phosphate group are retained on the column while non-phosphorylated and undesired contaminants pass through
- typical phosphoenriched protein yield is 5-10% of total protein
- contains materials required for six individual phosphoprotein enrichment procedures including six single-use columns, 100 ml 2X Binding/Wash Buffer, 30 ml Eluting Buffer, 150 µl each Phosphatase Inhibitor Cocktails 1 and 2, and six ultrafiltration concentrators for concentrating phosphoenriched protein post-purification
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関連項目
PhosphoCruz™ Protein Purification System 参考文献:
- Aurora-A 基因的高拷贝扩增与人类结直肠癌的染色体不稳定表型有关。 | Nishida, N., et al. 2007. Cancer Biol Ther. 6: 525-33. PMID: 17457043
订购信息
| 产品名称 | 产品编号 | 规格 | 价格 | 数量 | 收藏夹 | |
PhosphoCruz™ Protein Purification System | sc-24964 | 6 purifications | RMB2219.00 |