Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Pyruvate dehydrogenase kinase family members (PDK1, 2, 3, 4) are serine kinases that catalyze phosphorylation of the E1α subunit of the pyruvate dehydrogenase complex (PDC). PDC activity is controlled through phosphorylation and dephosphorylation of the E1α subunit, which leads to inactivation and reactivation, respectively. PDK3 binding to a free lipoyl domain (L2) in dihydrolypoyl acetyltransferase (E2), which comprises the core of PDC, leads to a large increase in E1α phosphorylation. Upregulation of PDK isoenzymes occurs during starvation conditions, where acetyl-CoA is alternatively generated through fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases in that both require histidine residues for activity. In mammals, transcripts for PDK3 are most abundant in testis and moderately expressed in heart and skeletal muscle.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
For Western Blot, is it recommended to use denatured or non-denatured conditions with PDK3 (RR-2): sc-100535 antibody?
Asked by: DefinitelyNotMatt
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.