PCSK1 抗体 (J-18): sc-100578

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Datasheets

- PCSK1 抗体 J-18 是小鼠单克隆 IgG₃ κ，PCSK1抗体, 在4篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组PCSK1
- 推荐用于 mouse, rat 和 human 来源的PCSK1 WB, IP, IF, IHC(P) 和 ELISA检测
- 关于如何获取PCSK1 (J-18): sc-100578的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG₃ BP-HRP 是PCSK1 Antibody (J-18) for WB and IHC(P)首选应用二抗. 这些试剂现在以套装形式提供PCSK1 Antibody (J-18) (见下方订购信息).

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此外 PCSK 抗体包括 PCSK1和 PCSK1N
Learn more about our ImmunoCruz® Antibody Conjugates and Cruz Marker™ MW Standards Promotional

PCSK1 Antibody (J-18) - Western Blotting - Image 33950

PCSK1 Antibody (J-18) - Immunohistochemistry - Image 36075

PCSK1 Antibody (J-18) - Immunofluorescence - Image 35599

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PCSK1 Antibody (J-18) is an IgG₃ κ mouse monoclonal PCSK1 antibody (also designated PCSK1 antibody) that detects the PCSK1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. PCSK1 Antibody (J-18) is available as the non-conjugated anti-PCSK1 antibody. PCSK1 (proprotein convertase subtilisin/kexin type 1), also known as PC1 (prohormone convertase 1), PC3, NEC1 (neuroendocrine convertase 1) or SPC3, is a member of the subtilisin-like proprotein convertase family that plays an important role in the processing of hormones such as Renin, Dynorphin, POMC, Synenkephalin, Insulin and Somatostatin. PCSK1 localizes to the cytoplasm and, using calcium as a cofactor, specifically catalyzes a hydrolysis reaction that releases protein hormones, Renin and neuropeptides from their corresponding precursors. PCSK1 is involved in the regulation of Insulin biosynthesis and functions as a type I proinsulin-processing enzyme. Mutations in the gene encoding PCSK1 can lead to PC1 deficiency, a disorder characterized by hypogonadism, obesity, reactive hypoglycemia, hypoadrenalism and small-intestinal absorptive dysfunction. Various isoforms exist for PCSK1 due to alternative splicing events.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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References:

The C-terminal region of proSAAS is a potent inhibitor of prohormone convertase 1. | Qian, Y., et al. 2000. J Biol Chem. 275: 23596-601. PMID: 10816562
The prohormone processing enzyme PC3 is a lipid raft-associated transmembrane protein. | Arnaoutova, I., et al. 2003. Biochemistry. 42: 10445-55. PMID: 12950171
Small-intestinal dysfunction accompanies the complex endocrinopathy of human proprotein convertase 1 deficiency. | Jackson, RS., et al. 2003. J Clin Invest. 112: 1550-60. PMID: 14617756
The cDNA sequence of the human pro-hormone and pro-protein convertase PC1. | Seidah, NG., et al. 1992. DNA Cell Biol. 11: 283-9. PMID: 1605851
MAFA controls genes implicated in insulin biosynthesis and secretion. | Wang, H., et al. 2007. Diabetologia. 50: 348-58. PMID: 17149590
Chromosomal assignments of the genes for neuroendocrine convertase PC1 (NEC1) to human 5q15-21, neuroendocrine convertase PC2 (NEC2) to human 20p11.1-11.2, and furin (mouse 7[D1-E2] region). | Seidah, NG., et al. 1991. Genomics. 11: 103-7. PMID: 1765368
Human prohormone convertase 3 gene: exon-intron organization and molecular scanning for mutations in Japanese subjects with NIDDM. | Ohagi, S., et al. 1996. Diabetes. 45: 897-901. PMID: 8666140
Proprotein convertase PC1/3-related peptides are potent slow tight-binding inhibitors of murine PC1/3 and Hfurin. | Boudreault, A., et al. 1998. J Biol Chem. 273: 31574-80. PMID: 9813073

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	PCSK1	Entrez Gene ID: 5122	5q15	NM_000439, NM_001177875, NM_001177876	UniProt ID: P29120	600955
Mouse	Pcsk1	Entrez Gene ID: 18548	13 C1	XM_006517153, XM_006517154, XM_006517155, NM_013628	UniProt ID: P63239	N/A

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗PCSK1抗体(J-18)在各种科学出版物中有4篇引文。

针对哺乳动物靶标蛋白的一抗，如抗PCSK1 抗体 (J-18)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

PCSK1 抗体 (J-18) Specifications

替代产品名称

PCSK1

产品克隆名称

J-18

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₃ kappa light chain

氨基酸

652-754

抗原表位靶标

PCSK1

抗原表位种属

human

产品反应性/检测

PCSK1

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, IHC(P), ELISA

瓶的信息

Each vial contains 100 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

4篇各种科学出版物的引文

可选的偶联物/形式

N/A

果蝇

非洲爪蟾蜍

拟南芥

秀丽隐杆线虫

梭菌

弯曲杆菌

幽门螺杆菌

肝炎

克雷白氏杆菌

结核分支杆菌

疟原虫

假单胞菌

酿酒酵母

沙门氏菌

血吸虫

志贺氏杆菌

葡萄球菌

Can sc-100578: PCSK1 (J-18) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp

Thank you for your question. Yes, sc-100578: PCSK1 (J-18) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 1 out of 5 by jerk from It doesn't work in IF in rat 832/13 ins1 cells PFA fixation, permeabilized with Triton X-100, used at 1:50

Date published: 2017-10-15

Rated 5 out of 5 by Randy McDonald from Strong IF staining We used this antibody for IF staining and got strong signaling from cytoplasm in HeLa cells

Date published: 2017-01-16

Rated 4 out of 5 by JL from Clear immunoperoxidase cytoplasmic staining Clear immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human small Intestine tissue. -SCBT QC

Date published: 2015-04-29

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PCSK1 抗体 (J-18): sc-100578

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References:

PCSK1 抗体 (J-18) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

Can sc-100578: PCSK1 (J-18) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?