PCMT1 抗体 (D-E7): sc-100977

PCMT1 Antibody (D-E7) is an IgG₁ κ mouse monoclonal PCMT1 antibody (also designated PCMT1 antibody) that detects the PCMT1 protein of mouse, rat and human origin by WB and IP. PCMT1 Antibody (D-E7) is available as the non-conjugated anti-PCMT1 antibody. PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase), also known as PIMT, is a member of the L-isoaspartyl/D-aspartyl protein methyltransferase family and is highly expressed in brain. Functioning as a monomer, PCMT localizes to the cytoplasm and participates in the degradation and/or repair of damaged proteins. More specifically, PCMT1 recognizes isomerized Asp or Asn residues in peptides and proteins and catalyzes the conversion of abnormal L-isoaspartyl and D-aspartyl residues to methyl esters that may then spontaneously hydrolyze to re-form normal aspartyl residues. In congruence with this reaction, PCMT1 converts the methyl donor S-adenosylmethionine (AdoMet) to S-andenosylhomocysteine (AdoHcy). In mice lacking PCMT1, damaged proteins accumulate in a variety of tissues and the ratio between AdoMet and AdoHcy is increased in brain tissue. The phenotypic result is progressive epilepsy and death at an early age.

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References:

1. Assignment of the protein L-isoaspartate (D-aspartate) O-methyltransferase gene (PCMT1) to human chromosome bands 6q24-->q25 with radiation hybrid mapping.  |  DeVry, CG. and Clarke, S. 1999. Cytogenet Cell Genet. 84: 130-1. PMID: 10343128
2. Polymorphic forms of the protein L-isoaspartate (D-aspartate) O-methyltransferase involved in the repair of age-damaged proteins.  |  DeVry, CG. and Clarke, S. 1999. J Hum Genet. 44: 275-88. PMID: 10496068
3. Altered levels of S-adenosylmethionine and S-adenosylhomocysteine in the brains of L-isoaspartyl (D-Aspartyl) O-methyltransferase-deficient mice.  |  Farrar, C. and Clarke, S. 2002. J Biol Chem. 277: 27856-63. PMID: 12023972
4. Alternative splicing of the human isoaspartyl protein carboxyl methyltransferase RNA leads to the generation of a C-terminal -RDEL sequence in isozyme II.  |  MacLaren, DC., et al. 1992. Biochem Biophys Res Commun. 185: 277-83. PMID: 1339271
5. The L-isoaspartyl/D-aspartyl protein methyltransferase gene (PCMT1) maps to human chromosome 6q22.3-6q24 and the syntenic region of mouse chromosome 10.  |  MacLaren, DC., et al. 1992. Genomics. 14: 852-6. PMID: 1478665
6. Diet-dependent survival of protein repair-deficient mice.  |  Farrar, CE. and Clarke, S. 2005. J Nutr Biochem. 16: 554-61. PMID: 16115544
7. Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase?  |  Wägner, AM., et al. 2007. Diabetologia. 50: 676-81. PMID: 17216280
8. Reactive oxygen species generated by thiol-modifying phenylarsine oxide stimulate the expression of protein L-isoaspartyl methyltransferase.  |  Fanεlus, I. and Desrosiers, RR. 2008. Biochem Biophys Res Commun. 371: 203-8. PMID: 18407833