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p53RFP Antibody (U-16) is an IgG2a κ mouse monoclonal p53RFP antibody (also designated p53RFP antibody) suitable for the detection of the p53RFP protein of human origin by WB, IP, IF and ELISA. p53RFP Antibody (U-16) is available as the non-conjugated anti-p53RFP antibody. p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I would like to know which epitope/protein region does it recognize? Thank you
Asked by: Abadcrt
Thank you for your question. This antibody, sc-101247, p53RFP (U-16), was raised against a recombinant p53RFP protein of human origin. The exact epitope has not been mapped, so we are unable to provide further information.
Answered by: Tech Service
Date published: 2019-12-10
I am using p53RFP (U-16): sc-101247 for immunofluorescence, what dilution of this antibody should I use?
Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: