m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Oval Cell Marker Antibody (OV-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with Oval Cell Marker Antibody (OV-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Oval Cell Marker Antibody (OV-6) is a high quality monoclonal Oval Cell Marker antibody (also designated Oval Cell Marker antibody) suitable for the detection of the Oval Cell Marker protein of mouse, rat and human origin. Oval Cell Marker Antibody (OV-6) is available as both the non-conjugated anti-Oval Cell Marker antibody form, as well as multiple conjugated forms of anti-Oval Cell Marker antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Stem cells are present in nearly all multi-cellular organisms and are characterized by their ability to self-renew and to differentiate into multiple different cell types. There are two broad types of stem cells, namely adult stem cells, which play an important role in tissue repair and organ maintenance, and embryonic stem cells, which are found in blastocysts and can differentiate to form any and all embryonic tissues. Due to their unique ability to mature into any cell type, stem cells are of particular interest in the treatment of human diseases, including cancer, muscle damage, spinal cord injuries, Parkinson′s disease, Amyotrophic laternal sclerosis and organ regeneration. Oval Cells are hepatic stem cells (stem cells that are liver-specific) that can differentiate into multiple cell types and are implicated in liver development, as well as in the pathogenesis of chronic liver diseases. Oval Cell Marker, also designated OV-6, can be used to detect the presence of Oval Cells within a particular sample.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Heloo. Please, write me what material can be used as a positive control of the product Oval Cell Marker Antibody (OV-6): sc-101863.
Asked by: Nino
Thank you for your question. Liver tissues and cells (hepatic samples) are considered the best positive controls. This antibody is specific for bile ducts, oval cells, some nodular hepatocytes and hepatomas.
Answered by: Tech Service
Date published: 2019-10-03
Which antigen retrieval technique is needed for IHC-P ?
Asked by: SophiePaquet
This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections, or for IHC(P). This antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-06-16
What negative control should be used when using the PE conjugated version of this antibody (sc-101863 PE) in flow cytometry?
Asked by: TinTin
Great question! We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. So if you are using sc-101863 PE, then you would want to use normal mouse IgG1 PE, sc-2866.