Oval Cell Marker 抗体 (OV-6): sc-101863

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Datasheets

- Oval Cell Marker 抗体 OV-6 是小鼠单克隆 IgG₁ κ，Oval Cell Marker抗体, 在7篇文献中引用，规格为200 µg/ml
- raised against carcinogen-treated nodular hepatocytes of rat origin
- Oval Cell Marker 抗体 (OV-6) 推荐用于 IF 和 FCM，检测mouse, rat 和human 来源的 OV-6 antigen
- 抗-Oval Cell Marker 抗体 (OV-6) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
- 还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
- 还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
- 关于如何获取Oval Cell Marker (OV-6): sc-101863的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP, m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 Oval Cell Marker Antibody (OV-6) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供Oval Cell Marker Antibody (OV-6)。 (见下方订购信息).

References:

The potential of stem cells. | Pfendler, KC. and Kawase, E. 2003. Obstet Gynecol Surv. 58: 197-208. PMID: 12612460
Embryonic stem cells as a cell source for treating Parkinson′s disease. | Fukuda, H. and Takahashi, J. 2005. Expert Opin Biol Ther. 5: 1273-80. PMID: 16197334
Stem cells--a clinical update. | Tuch, BE. 2006. Aust Fam Physician. 35: 719-21. PMID: 16969445
Embryonic stem cell transplantation: potential applicability in cell replacement therapy and regenerative medicine. | Wu, DC., et al. 2007. Front Biosci. 12: 4525-35. PMID: 17485394
Review: ex vivo engineering of living tissues with adult stem cells. | Barrilleaux, B., et al. 2006. Tissue Eng. 12: 3007-19. PMID: 17518617
Stem cell therapy for Parkinson′s disease. | Takahashi, J. 2007. Expert Rev Neurother. 7: 667-75. PMID: 17563250
Stem cells: the magic brew. | Rossant, J. 2007. Nature. 448: 260-2. PMID: 17637646
Stem cell transplantation: a promising therapy for Parkinson′s disease. | Wang, Y., et al. 2007. J Neuroimmune Pharmacol. 2: 243-50. PMID: 18040857
Embryonic stem cell lines derived from human blastocysts. | Thomson, JA., et al. 1998. Science. 282: 1145-7. PMID: 9804556

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Protocols Online

针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗Oval Cell Marker抗体(OV-6)在各种科学出版物中有7篇引文。

针对哺乳动物靶标蛋白的一抗，如抗Oval Cell Marker 抗体 (OV-6)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

Oval Cell Marker 抗体 (OV-6) Specifications

替代产品名称

Oval Cell Marker

产品克隆名称

OV-6

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

抗原表位靶标

Oval Cell Marker

抗原表位种属

rat

产品反应性/检测

OV-6 antigen

种属反应性/检测

mouse, rat, human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

IF, FCM

瓶的信息

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

7篇各种科学出版物的引文

可选的偶联物/形式

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗Oval Cell Marker抗体(OV-6)推荐用于以下应用：IF (Immunofluorescence), FCM (Flow Cytometry).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

Who knows which gene transcript OV6? I did not find the gene symbol OV6 in gene expression profiles

Asked by: drwang

Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.

Answered by: Technical Service SH

Date published: 2022-09-27

I'm getting unspecificity from this in mouse liver samples. I have tried diluting it more, different antigen retrievals and longer blocking times. Had a sample of this before which worked fine. Anyone know how to troubleshoot this?

Asked by: Sian

Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.

Answered by: Blake J

Date published: 2021-09-22

Heloo. Please, write me what material can be used as a positive control of the product Oval Cell Marker Antibody (OV-6): sc-101863. Thanks.

Asked by: Nino

Thank you for your question. Liver tissues and cells (hepatic samples) are considered the best positive controls. This antibody is specific for bile ducts, oval cells, some nodular hepatocytes and hepatomas.

Answered by: Tech Service

Date published: 2019-10-03

Which antigen retrieval technique is needed for IHC-P ? thanks

Asked by: SophiePaquet

This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections, or for IHC(P). This antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!

Answered by: Technical Support

Date published: 2019-06-16

What negative control should be used when using the PE conjugated version of this antibody (sc-101863 PE) in flow cytometry?

Asked by: TinTin

Great question! We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. So if you are using sc-101863 PE, then you would want to use normal mouse IgG1 PE, sc-2866.

Answered by: Technical Support 8

Date published: 2021-02-18

Rated 5 out of 5 by cjMara from Published for IF and IHC This mouse monoclonal Oval Cell Marker Antibody (OV-6) is featured in recent publications for use in IHC and IF research applications.

Date published: 2017-01-21

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