Oval Cell Marker抗体 (OV-6) 推荐用于 mouse, rat 和 human 来源的OV-6 antigen IF 和 FCM检测

Oval Cell Marker 抗体 (OV-6): sc-101863

Oval Cell Marker抗体 (OV-6) is rated 4.5 out of 5 by 2.
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Datasheets

    • 抗-Oval Cell Marker 抗体 OV-6 是小鼠单克隆 IgG1 κ,Oval Cell Marker抗体, 在5篇文献中引用,规格为200 µg/ml
    • raised against carcinogen-treated nodular hepatocytes of rat origin
    • 抗-Oval Cell Marker 抗体 (OV-6) 推荐用于 IF 和 FCM,检测mouse, rat 和human 来源的 OV-6 antigen
    • 抗-Oval Cell Marker 抗体 (OV-6) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Oval Cell Marker (OV-6): sc-101863免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Oval Cell Marker Antibody (OV-6) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供Oval Cell Marker Antibody (OV-6)。 (见下方订购信息).
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I'm getting unspecificity from this in mouse liver samples. I have tried diluting it more, different antigen retrievals and longer blocking times. Had a sample of this before which worked fine. Anyone know how to troubleshoot this?

Asked by: Sian
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-09-22

Heloo. Please, write me what material can be used as a positive control of the product Oval Cell Marker Antibody (OV-6): sc-101863. Thanks.

Asked by: Nino
Thank you for your question. Liver tissues and cells (hepatic samples) are considered the best positive controls. This antibody is specific for bile ducts, oval cells, some nodular hepatocytes and hepatomas.
Answered by: Tech Service
Date published: 2019-10-03

Which antigen retrieval technique is needed for IHC-P ? thanks

Asked by: SophiePaquet
This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections, or for IHC(P). This antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-06-16

What negative control should be used when using the PE conjugated version of this antibody (sc-101863 PE) in flow cytometry?

Asked by: TinTin
Great question! We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. So if you are using sc-101863 PE, then you would want to use normal mouse IgG1 PE, sc-2866.
Answered by: Technical Support 8
Date published: 2021-02-18
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Rated 5 out of 5 by from Published for IF and IHC This mouse monoclonal Oval Cell Marker Antibody (OV-6) is featured in recent publications for use in IHC and IF research applications.
Date published: 2017-01-21
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