NUDE1 抗体 (36-T): sc-100328

NUDE1 Antibody (36-T) is an IgG₁ κ mouse monoclonal NUDE1 antibody (also designated NUDE1 antibody) that detects the NUDE1 protein of human origin by WB, IP, IF and ELISA. NUDE1 Antibody (36-T) is available as the non-conjugated anti-NUDE1 antibody. NUDE1 (Nuclear Distribution Protein nudE homolog 1) is a 346 amino acid cytoplasmic protein belonging to the nudE protein family (whose members include NUDE1 and NDEL1). Phosphorylated during mitosis, NUDE1 is essential for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetochore proteins, as well as centrosome duplication during interphase. NUDE1 is thought to interact with NDEL1, LIS1 and Dynein IC1/2, cytosolic in a signaling pathway that regulates the formation of neurons and is fundamental to the development of the cerebral cortex. Mutations in the NUDE1 gene result in a reduced cerebral cortex size caused by defects in mitotic progression and chromosomal localization of cortical progenitors. NUDE1 is expressed as 2 isoforms produced by alternative splicing of the primary gene transcript.

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References:

1. Direct association of LIS1, the lissencephaly gene product, with a mammalian homologue of a fungal nuclear distribution protein, rNUDE.  |  Kitagawa, M., et al. 2000. FEBS Lett. 479: 57-62. PMID: 10940388
2. LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome.  |  Feng, Y., et al. 2000. Neuron. 28: 665-79. PMID: 11163258
3. Selective expression of doublecortin and LIS1 in developing human cortex suggests unique modes of neuronal movement.  |  Meyer, G., et al. 2002. Cereb Cortex. 12: 1225-36. PMID: 12427674
4. Human Nudel and NudE as regulators of cytoplasmic dynein in poleward protein transport along the mitotic spindle.  |  Yan, X., et al. 2003. Mol Cell Biol. 23: 1239-50. PMID: 12556484
5. Mitotic spindle regulation by Nde1 controls cerebral cortical size.  |  Feng, Y. and Walsh, CA. 2004. Neuron. 44: 279-93. PMID: 15473967
6. Subcellular targeting of DISC1 is dependent on a domain independent from the Nudel binding site.  |  Brandon, NJ., et al. 2005. Mol Cell Neurosci. 28: 613-24. PMID: 15797709
7. Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dynein-dependent and -independent centrosomal protein assembly.  |  Guo, J., et al. 2006. Mol Biol Cell. 17: 680-9. PMID: 16291865
8. Centrosomal proteins Nde1 and Su48 form a complex regulated by phosphorylation.  |  Hirohashi, Y., et al. 2006. Oncogene. 25: 6048-55. PMID: 16682949