Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
NFX1 Antibody (Y-14) is a high quality monoclonal NFX1 antibody (also designated NFX1 antibody) suitable for the detection of the NFX1 protein of human origin. NFX1 Antibody (Y-14) is available as the non-conjugated anti-NFX1 antibody. NFX1 (nuclear transcription factor, X-box binding 1), also known as NFX2, is a ubiquitously expressed nucleic acid binding protein. Localizing to the nucleus, NFX1 contains an R3H domain, a RING-type zinc finger and nine NFX1-type zinc fingers. NFX1 is induced by IFN-γ and functions as a transcriptional repressor, binding to the conserved X1 region within the X-box motif found in the promoter region of MHC class II genes. Acting as a potent repressor of MHC class II gene expression, NFX1 may be involved in regulating the duration of an inflammatory response. This suggests that NFX1 could be a useful target in the treatment of various diseases involving inflammation and autoimmunity. In addition, due to the presence of a RING-type finger domain, NFX1 may also function as an E3 ubiquitin-protein ligase. Two NFX1 isoforms, namely NFX1-91 and NFX1-123, exist due to alternative splicing events and differ in their C-termini.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using NFX1 (Y-14): sc-100973 for immunofluorescence, what dilution of this antibody should I use?
Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: