Neurogenin 1抗体 (OR-7) 推荐用于 mouse, rat 和 human 来源的Neurogenin 1 WB, IP, IF 和 ELISA检测

Neurogenin 1 抗体 (OR-7): sc-100332

Neurogenin 1抗体 (OR-7) is rated 3.0 out of 5 by 5.
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Datasheets

    • 抗-Neurogenin 1 抗体 OR-7 是小鼠单克隆 IgG2a (kappa轻链),Neurogenin 1抗体,规格为50 µg/0.5 ml
    • 免疫human物种的重组Neurogenin 1
    • 推荐用于 mouse, rat 和 human 来源的Neurogenin 1 WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Neurogenin 1 (OR-7): sc-100332免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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I am using Neurogenin 1 (OR-7): sc-100332 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24

Has anyone try this antibody for immunoprecipitation of endogenous protein? Thanks!

Asked by: Raquel
Thank you for your question. We are not aware of anyone publishing with Neurogenin 1 Antibody (OR-7): sc-100332 for immunoprecipitation. We do warranty the antibody to work for this research application based on the performance of similar antibodies and the reactivity of this antibody in Western blot. Please contact our Technical Service Department for further support. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Technical Support
Date published: 2017-01-19
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Rated 2 out of 5 by from no good signal in IF I didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 1 out of 5 by from Did not work for immunohistochemistry I tried this antibody at a 1:50 and 1:500 dilution, with or without TSA-based signal amplification, on adult mouse brain sections (using blocking buffer with 0.3% Triton-X100, over-night incubation of primary at 4'C). It didn't work.
Date published: 2018-01-18
Rated 4 out of 5 by from This has worked well in IHC This has worked well in IHC.
Date published: 2015-10-27
Rated 4 out of 5 by from Nice band seen in WB using HeLa nuclear extract Nice band seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-06-23
Rated 4 out of 5 by from Produced good immunofluorescence nuclear Produced good immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-02
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