Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The Wiskott-Aldrich syndrome (WAS) is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity, and a propensity for lymphoproliferative diseases. The syndrome is the result of a mutation in the gene encoding a proline-rich protein termed WASP. WASP and the related protein neural-WASP (or N-WASP) are downstream effectors of Cdc42. Both WASP and N-WASP are implicated in Actin polymerization and cytoskeletal organization, and N-WASP is also essential for mediating the Cdc42-induced formation of filopodia. WASP is primarily expressed in hematopoietic cells, whereas N-WASP is richest in neural tissues and is also expressed ubiquitously. The effects of Cdc42-stimulated Actin assembly require the interaction of WASP/N-WASP with the Arp2/3 complex, which dramatically enhances polymerization. The WASP and N-WASP proteins characteristically contain a pleckstrin homology (PH) domain, which binds phosphatidyl-inositol bisphosphate (PIP2); a Cdc42-binding domain; and a 70 amino acid conserved verprolin-homology (VPH) domain, which is the Actin-binding region and is critical to the regulation of the Actin cytoskeleton.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using N-WASP (93-W): sc-100964 for immunofluorescence, what dilution of this antibody should I use?
Asked by: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: