Myosin IIIa 抗体 (RR-8): sc-100953

Myosin IIIa Antibody (RR-8) is an IgG_2b κ mouse monoclonal Myosin IIIa antibody (also designated Myosin IIIa antibody) that detects the Myosin IIIa protein of mouse, rat and human origin by WB, IP and ELISA. Myosin IIIa Antibody (RR-8) is available as the non-conjugated anti-Myosin IIIa antibody. Myosins are highly conserved, ubiquitously expressed proteins that interact with Actin to generate the force for cellular movements. The human genome encodes over 40 different Myosin genes which are divided into distinct classes, the most notable of which are the conventional Myosins (class II) and the unconventional Myosins (classes I and III through XV). Myosin IIIA, also known as MYO3A or DFNB30, is a 1,616 amino acid class III Myosin that contains one protein kinase domain, one myosin head-like domain and three IQ domains. Highly expressed in pancreas, cochlea and retinal pigment cells, Myosin IIIA is thought to function as an Actin-based motor that may play a role in both vision and hearing. Additionally, via its protein kinase domain, Myosin IIIA is able to catalyze the ATP-dependent phosphorylation of a variety of target proteins. Defects in the gene encoding Myosin IIIA are the cause of non-syndromic sensorineural deafness autosomal recessive type 30 (DFNB30), a form of hearing loss that is caused by damage to neural receptors in the brain or inner ear.

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References:

1. Cloning and chromosomal localization of a human class III myosin.  |  Dosε, AC. and Burnside, B. 2000. Genomics. 67: 333-42. PMID: 10936054
2. From flies′ eyes to our ears: mutations in a human class III myosin cause progressive nonsyndromic hearing loss DFNB30.  |  Walsh, T., et al. 2002. Proc Natl Acad Sci U S A. 99: 7518-23. PMID: 12032315
3. Determination of human myosin III as a motor protein having a protein kinase activity.  |  Komaba, S., et al. 2003. J Biol Chem. 278: 21352-60. PMID: 12672820
4. Localization of a class III myosin to filopodia tips in transfected HeLa cells requires an actin-binding site in its tail domain.  |  Les Erickson, F., et al. 2003. Mol Biol Cell. 14: 4173-80. PMID: 14517327
5. Human myosin III is a motor having an extremely high affinity for actin.  |  Kambara, T., et al. 2006. J Biol Chem. 281: 37291-301. PMID: 17012748
6. A new compartment at stereocilia tips defined by spatial and temporal patterns of myosin IIIa expression.  |  Schneider, ME., et al. 2006. J Neurosci. 26: 10243-52. PMID: 17021180
7. Kinetic mechanism of human myosin IIIA.  |  Dosε, AC., et al. 2007. J Biol Chem. 282: 216-31. PMID: 17074769
8. The kinase domain alters the kinetic properties of the myosin IIIA motor.  |  Dosε, AC., et al. 2008. Biochemistry. 47: 2485-96. PMID: 18229949