MTSS1抗体 (M7-P3A7) 推荐用于 human 来源的MTSS1 WB, IP, IF 和 ELISA检测

MTSS1 抗体 (M7-P3A7): sc-101390

MTSS1抗体 (M7-P3A7) is rated 4.3 out of 5 by 3.
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Datasheets

    • 抗-MTSS1 抗体 M7-P3A7 是小鼠单克隆 IgG1 (kappa轻链),MTSS1抗体,规格为200 µg/ml
    • 免疫合成肽,对应于human来源MTSS1 679-688氨基酸
    • MTSS1 抗体 (M7-P3A7) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 MTSS1
    • 抗-MTSS1 抗体 (M7-P3A7) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取MTSS1 (M7-P3A7): sc-101390免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)

What is the recommended fixation method for immunofluorescence staining with MTSS1 (M7-P3A7): sc-101390 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from It works for western blot I could only say it works for western blot, when detecting recombinant MTSS1 overexpressed in HEK293 cells as the band is not so clear.
Date published: 2016-12-22
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmic Produced superb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-30
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