MRRF 抗体 (J-Q7): sc-100969

MRRF Antibody (J-Q7) is an IgG_2a κ mouse monoclonal MRRF antibody (also designated MRRF antibody) suitable for the detection of the MRRF protein of human origin by WB, IP, IF, IHC(P) and ELISA. MRRF Antibody (J-Q7) is available as the non-conjugated anti-MRRF antibody. The termination of protein synthesis is carried out by a variety of auxiliary factors that ensure the proper release of newly formed proteins. Once translation is complete, mRNA and P-site deacylated tRNA remain attached to the ribosome in a post-termination complex (post-TC) that must be dissociated and recycled in order for another round of translation to take place. MRRF (mitochondrial ribosome recycling factor), also known as RRF, MRFF or MTRRF, is a 262 amino acid protein that belongs to the RRF (ribosome recycling factor) family. Localized to mitochondria, MRRF is required for the release of ribosomes from mRNA at the end of protein biosynthesis. Via its ability to recycle ribosomes throughout translation, MRRF may actually increase overall translational efficiency, thereby playing an important role in the rate of protein synthesis. Multiple isoforms of MRRF are expressed due to alternative splicing events.

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References:

1. Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32-->q34.1 with radiation hybrid mapping.  |  Hansen, LL., et al. 2000. Cytogenet Cell Genet. 88: 91-2. PMID: 10773675
2. Binding of ribosome recycling factor to ribosomes, comparison with tRNA.  |  Hirokawa, G., et al. 2002. J Biol Chem. 277: 35847-52. PMID: 12138121
3. The role of ribosome recycling factor in dissociation of 70S ribosomes into subunits.  |  Hirokawa, G., et al. 2005. RNA. 11: 1317-28. PMID: 16043510
4. Progression of the ribosome recycling factor through the ribosome dissociates the two ribosomal subunits.  |  Barat, C., et al. 2007. Mol Cell. 27: 250-261. PMID: 17643374
5. Crystal structure of the ribosome recycling factor bound to the ribosome.  |  Weixlbaumer, A., et al. 2007. Nat Struct Mol Biol. 14: 733-7. PMID: 17660830
6. Recycling of eukaryotic posttermination ribosomal complexes.  |  Pisarev, AV., et al. 2007. Cell. 131: 286-99. PMID: 17956730
7. Structural Insights into ribosome recycling factor interactions with the 70S ribosome.  |  Pai, RD., et al. 2008. J Mol Biol. 376: 1334-47. PMID: 18234219
8. Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor.  |  Zhang, Y. and Spremulli, LL. 1998. Biochim Biophys Acta. 1443: 245-50. PMID: 9838146