Meis1/2/3 抗体 (9.2.7): sc-101850

Meis1/2/3 Antibody (9.2.7) is an IgG₁ κ mouse monoclonal Meis1/2/3 antibody (also designated Meis Homeobox antibody, MRG antibody, Meis1-Related Protein antibody, Homeobox Protein Meis antibody, Meis Myeloid Ecotropic Viral Integration Site 1 Homolog antibody, TALE Homeobox Protein Meis antibody, Meis1-Related Gene antibody, or Meis Homolog antibody) suitable for the detection of the Meis1/2/3 protein of mouse, rat and human origin by WB, IP, IF and IHC(P). Meis1/2/3 Antibody (9.2.7) is available as both the non-conjugated anti-Meis1/2/3 antibody form, as well as multiple conjugated forms of anti-Meis1/2/3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Hox, Pbx and Meis families of transcription factors form heteromeric complexes and bind DNA through specific homeobox domains. Hox proteins are involved in regulating tissue patterning during development, and are also expressed in lineage- and stage-specific patterns during adult hematopoietic differentiation and in leukemias. The Hox proteins, which include paralog groups 1-10, have a low intrinsic binding affinity for DNA and are instead associated into cooperative DNA binding complexes with Pbx or the Pbx- related Meis proteins, which result in an enhanced Hox-DNA binding affinity and an increased selectivity for the binding site. Both Meis1 and Meis2 (also known as Meis-related gene 1 or Mrg1) are members of the TALE ("three amino acid loop extension") family of homeodomain-containing proteins. In addition to binding with Hox proteins, Meis1 also forms heterodimers with the ubiquitously expressed Pbx proteins, including Pbx1, Pbx2 and Pbx3, and these complexes contain distinct DNA-binding specificities. Like Hox and Pbx proteins, Meis1 is implicated in oncogenesis, as it is overexpressed as a result of adjacent retroviral insertion in BHX-2 myeloid leukemias. Two Meis-related proteins, Meis2 and Meis3 (also designated Mrg1 and Mrg2, respectively), possess largely similar sequence identity with Meis1 and are expressed in normal tissues and myeloid leukemias. In the pancreas, Meis2 preferentially associates with Pbx1, and together they associate with the pancreas-specific homeodomain factor, Pdx1, to repress Pdx1-induced transcriptional activation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. PBX and MEIS as non-DNA-binding partners in trimeric complexes with HOX proteins.  |  Shanmugam, K., et al. 1999. Mol Cell Biol. 19: 7577-88. PMID: 10523646
2. Frequent co-expression of the HOXA9 and MEIS1 homeobox genes in human myeloid leukemias.  |  Lawrence, HJ., et al. 1999. Leukemia. 13: 1993-9. PMID: 10602420
3. Identification of a new family of Pbx-related homeobox genes.  |  Nakamura, T., et al. 1996. Oncogene. 13: 2235-42. PMID: 8950991
4. AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins.  |  Shen, WF., et al. 1997. Mol Cell Biol. 17: 6448-58. PMID: 9343407
5. Meis1 and pKnox1 bind DNA cooperatively with Pbx1 utilizing an interaction surface disrupted in oncoprotein E2a-Pbx1.  |  Knoepfler, PS., et al. 1997. Proc Natl Acad Sci U S A. 94: 14553-8. PMID: 9405651
6. Hoxa9 transforms primary bone marrow cells through specific collaboration with Meis1a but not Pbx1b.  |  Kroon, E., et al. 1998. EMBO J. 17: 3714-25. PMID: 9649441
7. An endocrine-exocrine switch in the activity of the pancreatic homeodomain protein PDX1 through formation of a trimeric complex with PBX1b and MRG1 (MEIS2).  |  Swift, GH., et al. 1998. Mol Cell Biol. 18: 5109-20. PMID: 9710595