Med15 抗体 (KJ-2): sc-101185

Med15 Antibody (KJ-2) is an IgG_2a κ mouse monoclonal Med15 antibody (also designated Med15 antibody) that detects the Med15 protein of mouse, rat and human origin by WB, IP, IF and ELISA. Med15 Antibody (KJ-2) is available as the non-conjugated anti-Med15 antibody. In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The Mediator complex is one such multiprotein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med15 (Mediator of RNA polymerase II transcription subunit 15), also known as ARC105, CTG7A, PCQAP, TIG1 or TNRC7, is a 788 amino acid subunit of the Mediator complex that localizes to both the nucleus and the cytoplasm. Expressed ubiquitously with highest expression in placenta and blood, Med15 participates in the regulation of Pol II-mediated gene expression and is thought to play a key role in the control of lipid homeostatsis. The gene encoding Med15 is located in a region on chromosome 22 that is deleted in DiGeorge syndrome, suggesting that the loss of Med15 may be associated with this rare congenital disease. Due to alternative splicing events, Med15 is expressed as two isoforms.

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References:

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2. A novel glutamine-rich putative transcriptional adaptor protein (TIG-1), preferentially expressed in placental and bone-marrow tissues.  |  Abraham, S. and Solomon, WB. 2000. Gene. 255: 389-400. PMID: 11024300
3. Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunit.  |  Berti, L., et al. 2001. Genomics. 74: 320-32. PMID: 11414760
4. A component of the ARC/Mediator complex required for TGF beta/Nodal signalling.  |  Kato, Y., et al. 2002. Nature. 418: 641-6. PMID: 12167862
5. Methylation of the retinoid response gene TIG1 in prostate cancer correlates with methylation of the retinoic acid receptor beta gene.  |  Zhang, J., et al. 2004. Oncogene. 23: 2241-9. PMID: 14691453
6. An association study of PCQAP polymorphisms and schizophrenia.  |  Sandhu, HK., et al. 2004. Psychiatr Genet. 14: 169-72. PMID: 15318033
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8. An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis.  |  Yang, F., et al. 2006. Nature. 442: 700-4. PMID: 16799563
9. TRIM11 binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105) through the ubiquitin-proteasome system.  |  Ishikawa, H., et al. 2006. FEBS Lett. 580: 4784-92. PMID: 16904669