Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
MAML2 Antibody (G-07) is a high quality monoclonal MAML2 antibody (also designated MAML2 antibody) suitable for the detection of the MAML2 protein of human origin. MAML2 Antibody (G-07) is available as the non-conjugated anti-MAML2 antibody. MAML2 (Mastermind-like protein 2), also known as MAM2, MAM3 or MLL-MAML2, is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
For Western Blot, is it recommended to use denatured or non-denatured conditions with MAML2 (G-07): sc-100778 antibody?
Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Rated 2 out of
Cross reacted with other proteins?1:500 dilution ON 4 deg C in TBS/BSA on western of N2a whole cell lysate. Got a very strong band around 50 KDa (tubulin?). Tried cutting the membrane (to JUST the high MW) in case cross-reactivity was diluting out true signal but still got nothin...two stars cuz it was free and to be fair, the 50KDa signal was really good...whatever it was...