MAK 抗体 (JK-10): sc-100432

MAK Antibody (JK-10) is an IgG_2a κ mouse monoclonal MAK antibody (also designated MAK antibody) that detects the MAK protein of human origin by WB, IP, IF and ELISA. MAK Antibody (JK-10) is available as the non-conjugated anti-MAK antibody. The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. MAK (male germ cell-associated kinase) is a 623 amino acid member of the Ser/Thr protein kinase family. Expressed predominantly in testicular germ cells, MAK contains one protein kinase domain and is believed to play an important role in spermatogenesis, as it is involved in the regulation of cell cycle and cell fate. MAK is a homolog of the S. cerevisiae protein Ime2, a meiosis-specific protein kinase that is required for the initiation of meiosis and spore formation. MAK expression is induced by androgen and MAK physically associates with AR (androgen receptor), functioning as a coactivator. The knockdown of MAK expression results in diminished expression of AR-responsive genes and inhibition of androgen-induced growth.

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References:

1. A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation.  |  Shinkai, Y., et al. 2002. Mol Cell Biol. 22: 3276-80. PMID: 11971961
2. CK2 controls multiple protein kinases by phosphorylating a kinase-targeting molecular chaperone, Cdc37.  |  Miyata, Y. and Nishida, E. 2004. Mol Cell Biol. 24: 4065-74. PMID: 15082798
3. Activation of a nuclear Cdc2-related kinase within a mitogen-activated protein kinase-like TDY motif by autophosphorylation and cyclin-dependent protein kinase-activating kinase.  |  Fu, Z., et al. 2005. Mol Cell Biol. 25: 6047-64. PMID: 15988018
4. Androgen receptor-dependent PSA expression in androgen-independent prostate cancer cells does not involve androgen receptor occupancy of the PSA locus.  |  Jia, L. and Coetzee, GA. 2005. Cancer Res. 65: 8003-8. PMID: 16140973
5. Male germ cell-associated kinase, a male-specific kinase regulated by androgen, is a coactivator of androgen receptor in prostate cancer cells.  |  Ma, AH., et al. 2006. Cancer Res. 66: 8439-47. PMID: 16951154
6. Identification of yin-yang regulators and a phosphorylation consensus for male germ cell-associated kinase (MAK)-related kinase.  |  Fu, Z., et al. 2006. Mol Cell Biol. 26: 8639-54. PMID: 16954377
7. A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis.  |  Matsushime, H., et al. 1990. Mol Cell Biol. 10: 2261-8. PMID: 2183027
8. Mouse Mak gene for male germ cell-associated kinase maps to chromosome 13.  |  Taketo, M., et al. 1994. Genomics. 19: 397-8. PMID: 8188277